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验证数据展示

  • WB analysis using 68561-1-Ig (same clone as 68561-1-PBS)

    WB analysis using 68561-1-Ig (same clone as 68561-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68561-1-Ig (Integrin alpha-5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68561-1-PBS in a different storage buffer formulation.

  • WB analysis of HAP1 using 68561-1-Ig (same clone as 68561-1-PBS)

    WB analysis of HAP1 using 68561-1-Ig (same clone as 68561-1-PBS)

    WB result of Integrin alpha-5/CD49e antibody (68561-1-Ig; 1:8000; room temperature for 1.5 hours) with wild-type and Integrin alpha-5/CD49e knockout HAP1 cells. This data was developed using the same antibody clone with 68561-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

Integrin alpha-5/CD49e Monoclonal antibody, PBS Only

Integrin alpha-5/CD49e Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68561-1-PBS

产品说明书

CloneNo. 3B9E5

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, Indirect ELISA

别名

Integrin Alpha-5, ITGA5, 3B9E5, CD49 antigen-like family member E, CD49e

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ITGA5 Ready-To-Use IHC Kit. ITGA5 primary antibody included.

产品信息

68561-1-PBS targets Integrin alpha-5/CD49e in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原

CatNo: Ag33642

Product name: Recombinant human Integrin alpha-5 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 749-1049 aa of BC008786

Sequence: FTVPHLRDTKKTIQFDFQILSKNLNNSQSDVVSFRLSVEAQAQVTLNGVSKPEAVLFPVSDWHPRDQPQKEEDLGPAVHHVYELINQGPSSISQGVLELSCPQALEGQQLLYVTRVTGLNCTTNHPINPKGLELDPEGSLHHQQKREAPSRSSASSGPQILKCPEAECFRLRCELGPLHQQESQSLQLHFRVWAKTFLQREHQPFSLQCEAVYKALKMPYRILPRQLPQKERQVATAVQWTKAEGSYGVPLWIIILAILFGLLLLGLLIYILYKLGFFKRSLPYGTAMEKAQLKPPATSDA

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
别名 Integrin Alpha-5, ITGA5, 3B9E5, CD49 antigen-like family member E, CD49e
计算分子量 115 kDa
观测分子量140 kDa
GenBank蛋白编号BC008786
基因名称 Integrin alpha 5
Gene ID (NCBI) 3678
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP08648
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin alpha 5 (ITGA5), belongs to the integrin alpha chain family, primarily binds to Integrin beta 1 (ITGB1) to form an α5β1 heterodimer (PMID: 33711961). Integrin α5β1 is a specific receptor of fibronectin through its arginine-glycine-aspartic acid (RGD) binding site (PMID: 19608542). Integrin α5β1 plays roles in cell adhesion, migration and matrix formation, and has emerged as an essential mediator in many human carcinomas (PMID: 33408483).

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