Integrin alpha-5 Monoclonal antibody
Integrin alpha-5 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, Mouse, Rat, Pig
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
3B9E5
验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, rat colon tissue, K-562 cells, Jurkat cells, pig colon tissue, HeLa cells, mouse colon tissue, mouse small intestine tissue |
Planning an IHC experiment? We recommend our IHCeasy ITGA5 Ready-To-Use IHC Kit. ITGA5 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68561-1-Ig targets Integrin alpha-5 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.
| Tested Applications | WB, ELISA |
| Tested Reactivity | Human, Mouse, Rat, Pig |
| Immunogen | Integrin alpha-5 fusion protein Ag33642 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | integrin, alpha 5 (fibronectin receptor, alpha polypeptide) |
| Synonyms | CD49e, FNRA, Integrin alpha 5, Integrin alpha F, ITGA5, VLA 5, VLA5A |
| Calculated Molecular Weight | 115 kDa |
| Observed Molecular Weight | 140 kDa |
| GenBank Accession Number | BC008786 |
| Gene Symbol | ITGA5 |
| Gene ID (NCBI) | 3678 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P08648 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin alpha 5 (ITGA5), belongs to the integrin alpha chain family, primarily binds to Integrin beta 1 (ITGB1) to form an α5β1 heterodimer (PMID: 33711961). Integrin α5β1 is a specific receptor of fibronectin through its arginine-glycine-aspartic acid (RGD) binding site (PMID: 19608542). Integrin α5β1 plays roles in cell adhesion, migration and matrix formation, and has emerged as an essential mediator in many human carcinomas (PMID: 33408483).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Integrin alpha-5 antibody 68561-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
