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验证数据展示

  • WB analysis using 87056-2-RR (same clone as 87056-2-PBS)

    WB analysis using 87056-2-RR (same clone as 87056-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87056-2-RR (Integrin alpha-1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87056-2-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 87056-2-RR (same clone as 87056-2-PBS)

    IF Staining of HeLa using 87056-2-RR (same clone as 87056-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Integrin alpha 1 antibody (87056-2-RR, Clone: 251984B7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87056-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP02854-2

    Sandwich ELISA sample test of MP02854-2

    The mean Integrin alpha 1 concentration was determined to be 241.89 ng/mL in HepG2 cell extract based on a 1.20 mg/mL extract load, 523.16 ng/mL in HuH-7 cell extract based on a 1.20 mg/mL extract load, 15.42 ng/mL in K-562 cell extract based on a 1.20 mg/mL extract load and 17.42 ng/mL in U-937 cell extract based on a 1.20 mg/mL extract load.

  • Sandwich ELISA sample test of MP02854-2

    Sandwich ELISA sample test of MP02854-2

    Plasma of eight individual healthy human donors was measured. The Integrin alpha 1 concentration of detected samples was determined to be 84.94 ng/mL with a range of 50.93-128.02 ng/mL.

  • Sandwich ELISA standard curve of MP02854-2

    Sandwich ELISA standard curve of MP02854-2

    Sandwich ELISA standard curve of MP02854-2, Human Integrin alpha 1 Recombinant Matched Antibody Pair - PBS only. 87056-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2089. 87056-4-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

  • Cytometric bead array sample test of MP02854-1

    Cytometric bead array sample test of MP02854-1

    The mean Integrin alpha-1 concentration was determined to be 51.4 ng/mL in human plasma.

  • Cytometric bead array standard curve of MP02854-1

    Cytometric bead array standard curve of MP02854-1

    Cytometric bead array standard curve of MP02854-1, Integrin alpha-1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 87056-2-PBS. Detection antibody: 87056-1-PBS. Standard: Eg2089. Range: 0.781-100 ng/mL.

  • Affinity of 87056-2-PBS

    Affinity of 87056-2-PBS

    Biolayer interferometry (BLl) kinetic assay of 87056-2-PBS against Human Integrin alpha-1 was performed. The affinity constant is 1.16 nM.

  • Affinity of 87056-2-RR

    Affinity of 87056-2-RR

    Biolayer interferometry (BLl) kinetic assays of 87056-2-RR against Human Integrin alpha 1 were performed. The affinity constant is 0.468 nM.

Integrin alpha 1 Recombinant monoclonal antibody, PBS Only (Capture)

Integrin alpha 1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA
Cat No. 87056-2-PBS

产品说明书

CloneNo. 251984B7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

别名

ITGA1, CD49 antigen-like family member A, CD49a, integrin, alpha 1, Laminin and collagen receptor

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy Integrin alpha-1 Ready-To-Use IHC Kit. Integrin alpha-1 primary antibody included.

产品信息

87056-2-PBS targets Integrin alpha 1 as part of a matched antibody pair:

MP02854-1: 87056-2-PBS capture and 87056-1-PBS detection (validated in Cytometric bead array)

MP02854-2: 87056-2-PBS capture and 87056-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg2089

Product name: Recombinant Human Integrin alpha-1 protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 29-1141 aa of BC137121

Sequence: FNVDVKNSMTFSGPVEDMFGYTVQQYENEEGKWVLIGSPLVGQPKNRTGDVYKCPVGRGESLPCVKLDLPVNTSIPNVTEVKENMTFGSTLVTNPNGGFLACGPLYAYRCGHLHYTTGICSDVSPTFQVVNSIAPVQECSTQLDIVIVLDGSNSIYPWDSVTAFLNDLLERMDIGPKQTQVGIVQYGENVTHEFNLNKYSSTEEVLVAAKKIVQRGGRQTMTALGIDTARKEAFTEARGARRGVKKVMVIVTDGESHDNHRLKKVIQDCEDENIQRFSIAILGSYNRGNLSTEKFVEEIKSIASEPTEKHFFNVSDELALVTIVKTLGERIFALEATADQSAASFEMEMSQTGFSAHYSQDWVMLGAVGAYDWNGTVVMQKASQIIIPRNTTFNVESTKKNEPLASYLGYTVNSATASSGDVLYIAGQPRYNHTGQVIIYRMEDGNIKILQTLSGEQIGSYFGSILTTTDIDKDSNTDILLVGAPMYMGTEKEEQGKVYVYALNQTRFEYQMSLEPIKQTCCSSRQHNSCTTENKNEPCGARFGTAIAAVKDLNLDGFNDIVIGAPLEDDHGGAVYIYHGSGKTIRKEYAQRIPSGGDGKTLKFFGQSIHGEMDLNGDGLTDVTIGGLGGAALFWSRDVAVVKVTMNFEPNKVNIQKKNCHMEGKETVCINATVCFDVKLKSKEDTIYEADLQYRVTLDSLRQISRSFFSGTQERKVQRNITVRKSECTKHSFYMLDKHDFQDSVRITLDFNLTDPENGPVLDDSLPNSVHEYIPFAKDCGNKEKCISDLSLHVATTEKDLLIVRSQNDKFNVSLTVKNTKDSAYNTRTIVHYSPNLVFSGIEAIQKDSCESNHNITCKVGYPFLRRGEMVTFKILFQFNTSYLMENVTIYLSATSDSEEPPETLSDNVVNISIPVKYEVGLQFYSSASEYHISIAANETVPEVINSTEDIGNEINIFYLIRKSGSFPMPELKLSISFPNMTSNGYPVLYPTGLSSSENANCRPHIFEDPFSINSGKKMTTSTDHLKRGTILDCNTCKFATITCNLTSSDISQVNVSLILWKPTFIKSYFSSLNLTIRGELRSENASLVLSSSNQKRELAIQISKDGLPGRVP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 integrin, alpha 1
别名 ITGA1, CD49 antigen-like family member A, CD49a, integrin, alpha 1, Laminin and collagen receptor
计算分子量 1179 aa, 131 kDa
观测分子量200 kDa
GenBank蛋白编号BC137121
基因名称 Integrin alpha 1
Gene ID (NCBI) 3672
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP56199
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID:19118207). Integrin alpha-1 (ITGA1, CD49a) combines with the beta 1 subunit (ITGB1, CD29) to form a cell-surface receptor for collagen and laminin. This receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. Integrin alpha-1 is a transmembrane glycoprotein with an apparent molecular weight of 180-250 kDa, larger than the calculated molecular weight of 131 kDa (PMID: 2475259; 3257425; 11557277; 18840653).

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