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验证数据展示

  • WB analysis using 84883-5-RR (same clone as 84883-5-PBS)

    WB analysis using 84883-5-RR (same clone as 84883-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84883-5-RR (Integrin Alpha V antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84883-5-PBS in a different storage buffer formulation.

  • IHC staining of human hepatocellular cancer using 84883-5-RR (same clone as 84883-5-PBS)

    IHC staining of human hepatocellular cancer using 84883-5-RR (same clone as 84883-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocellular cancer slide using 84883-5-RR (Integrin Alpha V antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84883-5-PBS in a different storage buffer formulation.

  • IHC staining of human hepatocellular cancer using 84883-5-RR (same clone as 84883-5-PBS)

    IHC staining of human hepatocellular cancer using 84883-5-RR (same clone as 84883-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocellular cancer slide using 84883-5-RR (Integrin Alpha V antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84883-5-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 84883-5-RR (same clone as 84883-5-PBS)

    FC experiment of A549 using 84883-5-RR (same clone as 84883-5-PBS)

    1x10^6 A549 cells were surface stained with 0.25 ug Integrin Alpha V Recombinant monoclonal antibody (84883-5-RR, Clone: 242376D3) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Cells were not fixed. This data was developed using the same antibody clone with 84883-5-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 84883-5-RR (same clone as 84883-5-PBS)

    IF Staining of A549 using 84883-5-RR (same clone as 84883-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using Integrin Alpha V antibody (84883-5-RR, Clone: 242376D3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84883-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84883-5-RR

    Affinity and Kinetic Characterization of 84883-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84883-5-RR against Human Integrin Alpha V were performed. The affinity constant is 0.121 nM.

Integrin Alpha V Recombinant monoclonal antibody, PBS Only

Integrin Alpha V Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC, Indirect ELISA
Cat No. 84883-5-PBS

产品说明书

CloneNo. 242376D3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC, Indirect ELISA

别名

ITGAV, 242376D3, CD51, Integrin alpha-V, Integrin alpha-V heavy chain

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84883-5-PBS targets Integrin Alpha V in WB, IHC, IF/ICC, FC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag25825

Product name: Recombinant human ITGAV protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 819-915 aa of BC136442

Sequence: SFSKAMLHLQWPYKYNNNTLLYILHYDIDGPMNCTSDMEINPLRIKISSLQTTEKNDTVAGQGERDHLITKRDLALSEGDIHTLGCGVAQCLKIVCQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)
别名 ITGAV, 242376D3, CD51, Integrin alpha-V, Integrin alpha-V heavy chain
计算分子量 1048 aa, 116 kDa
观测分子量130 kDa
GenBank蛋白编号BC136442
基因名称 Integrin alpha V
Gene ID (NCBI) 3685
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP06756
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin alpha V, also known as CD51, is a type I transmembrane glycoprotein composed of an extracellular domain, a transmembrane region and cytoplasmic domain (PMID: 2430295; 1690718). It can form heterodimers with one of the five beta integrin subunits (beta 1, 3, 5, 6, 8) that recognize ligands containing the sequence Arg-Gly-Asp, such as vitronectin, fibronectin, and osteopontin (PMID: 37087799). Integrin alpha V mainly plays a role in extracellular matrix-mediated cell adhesion and migration, differentiation, wound healing, inflammation, tumorigenesis, proliferation, angiogenesis, and metastasis.

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