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验证数据展示

  • IHC staining of rat pancreas using 67284-1-Ig

    IHC staining of rat pancreas using 67284-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 67284-1-Ig (Insulin1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat pancreas using 67284-1-Ig

    IHC staining of rat pancreas using 67284-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 67284-1-Ig (Insulin1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of rat pancreas using 67284-1-Ig

    IF Staining of rat pancreas using 67284-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed rat pancreas tissue using Ins1 antibody (67284-1-Ig, Clone: 3A11C1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Ins1 Monoclonal antibody

Ins1 Monoclonal Antibody for IHC, IF-P, ELISA
Cat No. 67284-1-Ig

产品说明书

CloneNo. 3A11C1

宿主/亚型

Mouse / IgG2a

种属反应性

Rat, mouse

应用

IHC, IF-P, ELISA

别名

Ins1, Ins-1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inrat pancreas tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat pancreas tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:5000-1:10000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67284-1-Ig targets Ins1 in IHC, IF-P, ELISA applications and shows reactivity with Rat, mouse samples.

经测试应用 IHC, IF-P, ELISA Application Description
经测试反应性 Rat, mouse
免疫原 Ins1 fusion protein Ag28809 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 insulin 1
别名 Ins1, Ins-1
计算分子量 12 kDa
GenBank蛋白编号NM_019129.3
基因名称 Ins1
Gene ID (NCBI) 24505
RRIDAB_2882551
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP01322
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for Ins1 antibody 67284-1-IgDownload protocol
IF protocol for Ins1 antibody 67284-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols