×
Use Able AI chat for product recommendations

验证数据展示

  • FC experiment of human PBMCs using 98744-1-RR

    FC experiment of human PBMCs using 98744-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human Human IgM Rabbit RecAb (98744-1-RR, Clone: 252244H7) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Human IgD. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • IHC staining of human tonsillitis using 98744-1-RR

    IHC staining of human tonsillitis using 98744-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 98744-1-RR (IgM antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with human IgM antibody included
  • FC experiment of human PBMCs using 98744-1-RR

    FC experiment of human PBMCs using 98744-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human Human IgM Rabbit RecAb (98744-1-RR, Clone: 252244H7) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • IHC staining of human tonsillitis using 98744-1-RR

    IHC staining of human tonsillitis using 98744-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 98744-1-RR (IgM antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with human IgM antibody included

Anti-Human IgM Rabbit Recombinant Antibody

IgM Uni-rAb® Recombinant Antibody for IHC, FC
Cat No. 98744-1-RR

产品说明书

CloneNo. 252244H7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, FC

别名

human IgM, IGHM, MU, VH

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy human IgM Ready-To-Use IHC Kit. human IgM primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98744-1-RR targets IgM in IHC, FC applications and shows reactivity with human samples.

经测试应用 IHC, FC Application Description
经测试反应性 human
免疫原

CatNo: Eg6742

Product name: Recombinant Human IgM protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 105-448 aa of BC020240

Sequence: VIAELPPKVSVFVPPRDGFFGNPRKSKLICQATGFSPRQIQVSWLREGKQVGSGVTTDQVQAEAKESGPTTYKVTSTLTIKESDWLGQSMFTCRVDHRGLTFQQNASSMCVPDQDTAIRVFAIPPSFASIFLTKSTKLTCLVTDLTTYDSVTISWTRQNGEAVKTHTNISESHPNATFSAVGEASICEDDWNSGERFTCTVTHTDLPSPLKQTISRPKGVALHRPDVYLLPPAREQLNLRESATITCLVTGFSPADVFVQWMQRGQPLSPEKYVTSAPMPEPQAPGRYFAHSILTVSEEEWNTGETYTCVVAHEALPNRVTERTVDKSTEGEVSADEEGFENLW

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 immunoglobulin heavy constant mu
别名 human IgM, IGHM, MU, VH
GenBank蛋白编号BC020240
基因名称 IGHM
Gene ID (NCBI) 3507
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

IgM (Immunoglobulin M) is the first immunoglobulin expressed during B cell development. IgM antibodies play an important role in primary immune response involved in early recognition of external invaders like bacteria and viruses, cellular waste and modified self, as well as in recognition and elimination of precancerous and cancerous lesions.

实验方案

Product Specific Protocols
FC protocol for IgM antibody 98744-1-RRDownload protocol
IHC protocol for IgM antibody 98744-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat

Able™

正在加载，请稍候...