验证数据展示
经过测试的应用
| Positive FC detected in | human PBMCs, rat splenocytes, THP-1 cells |
For other applications, we recommend the unconjugated version of this antibody, 98740-1-MR
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-98740 targets IgG2b, κ Isotype Control in FC applications and shows reactivity with n/a samples.
| 经测试应用 | FC Application Description |
| 经测试反应性 | n/a |
| 免疫原 |
unknow 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | IgG2b, κ Isotype Control |
| 别名 | IgG2b Isotype Control, Ighg2b, gamma2b, IgG2b, Igh-3 |
| 基因名称 | |
| Gene ID (NCBI) | |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 激发激光 | Blue laser (488 nm) |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
This product is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 IgG2b, κ Isotype Control antibody CL488-98740 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
