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1X10^6 BALB/c mouse splenocytes were surface stained with 0.5 ug Rat IgG2b Isotype Control (65211-1-Ig, Clone: LTF-2) (blue) and Fluorescein (FITC)-conjugated Affinipure Goat Anti-Rat IgG(H+L) at dilution of 1:1000, or with 0.5 ug Anti-Mouse CD4 (65104-1-Ig, Clone: GK1.5) (red) and Fluorescein (FITC)-conjugated Affinipure Goat Anti-Rat IgG(H+L) at dilution of 1:1000. Cells were not fixed.
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
The LTF-2 immunoglobulin is useful as an isotype-matched control. The LTF-2 immunolglobulin has an unknown binding specificity and is used as an isotype control for rat IgG2b antibodies. This antibody has been quality-tested for flow cytometry as negative control.