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验证数据展示

  • FC experiment of human PBMCs using 98741-1-MR (same clone as 98741-1-PBS)

    FC experiment of human PBMCs using 98741-1-MR (same clone as 98741-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Mouse IgG1, λ isotype control RecAb (98741-1-MR, Clone:253505A1) (red in histogram) and PE-conjugated Goat Anti-Mouse IgG, or 0.25 ug Anti-Human CD4 (RPA-T4) (65143-1-Ig, Clone: RPA-T4) (green in histogram), or unstained cells (blue in histogram). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98741-1-PBS in a different storage buffer formulation.

  • FC experiment of rat splenocytes using 98741-1-MR (same clone as 98741-1-PBS)

    FC experiment of rat splenocytes using 98741-1-MR (same clone as 98741-1-PBS)

    1x10^6 rat splenocytes were surface stained with 0.25 ug Mouse IgG1, λ isotype control RecAb (98741-1-MR, Clone:253505A1) (red in histogram) and PE-conjugated Goat Anti-Mouse IgG, or 0.25 ug Anti-Rat CD45 (OX1) (65228-1-Ig, Clone: OX1) (green in histogram), or unstained cells (blue in histogram). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98741-1-PBS in a different storage buffer formulation.

Mouse IgG1, λ Isotype Control Recombinant Antibody, PBS Only

IgG1, λ Isotype Control Uni-rAb® Recombinant Antibody for FC
Cat No. 98741-1-PBS

产品说明书

CloneNo. 253505A1

宿主/亚型

Mouse / IgG1

种属反应性

n/a

应用

FC

别名

Mouse IgG1 λ Isotype Control, IgG1 Isotype Control, Mouse IgG1 isotype control

缓冲液配方:  PBS Only
PBS, Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

98741-1-PBS targets IgG1, λ Isotype Control in FC applications and shows reactivity with n/a samples.

经测试应用 FC Application Description
经测试反应性 n/a
免疫原

unknow

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Recombinant
产品类型 Antibody
全称 IgG1, λ Isotype Control
别名 Mouse IgG1 λ Isotype Control, IgG1 Isotype Control, Mouse IgG1 isotype control
基因名称
Gene ID (NCBI)
RRIDAB_3746308
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

An IgG1, λ isotype control is a critical negative control reagent used in flow cytometry to distinguish specific antibody binding from non-specific background signals. It is an antibody that shares the same host species, immunoglobulin class (IgG1), light chain type (λ), and fluorophore conjugate as the primary antibody used in an experiment, but it lacks specificity for the target antigen.

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