验证数据展示
经过测试的应用
| Positive FC detected in | human PBMCs, rat splenocytes |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
98741-1-MR targets IgG1, λ Isotype Control in FC applications and shows reactivity with n/a samples.
| 经测试应用 | FC Application Description |
| 经测试反应性 | n/a |
| 免疫原 |
unknow 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | IgG1, λ Isotype Control |
| 别名 | Mouse IgG1 λ Isotype Control, IgG1 Isotype Control, Mouse IgG1 isotype control |
| 基因名称 | |
| Gene ID (NCBI) | |
| RRID | AB_3746308 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2 - 8°C. Stable for one year after shipment. |
背景介绍
An IgG1, λ isotype control is a critical negative control reagent used in flow cytometry to distinguish specific antibody binding from non-specific background signals. It is an antibody that shares the same host species, immunoglobulin class (IgG1), light chain type (λ), and fluorophore conjugate as the primary antibody used in an experiment, but it lacks specificity for the target antigen.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for IgG1, λ Isotype Control antibody 98741-1-MR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
