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验证数据展示

  • FC experiment of HeLa using CL647-30000

    FC experiment of HeLa using CL647-30000

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Rabbit IgG control (CL647-30000) (red), or 0.2 ug CoraLite® Plus 647-conjugated TRAP1 Rabbit PolyAb (CL647-10325) (green), or unstained (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 647-conjugated Rabbit IgG control Polyclonal antibody

Rabbit IgG control Polyclonal Antibody for FC (Intra)
Cat No. CL647-30000

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

n/a

应用

FC (Intra)

别名

Rabbit IgG, Rabbit Immunoglobulin, Rabbit IgG isotype, Isotype control, IgG control

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  {{ptg:cur_Conjugation}}
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-30000 targets Rabbit IgG control in FC (Intra) applications and shows reactivity with n/a samples.

经测试应用 FC (Intra) Application Description
经测试反应性 n/a
免疫原 N/A 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Rabbit IgG control
别名 Rabbit IgG, Rabbit Immunoglobulin, Rabbit IgG isotype, Isotype control, IgG control
基因名称
Gene ID (NCBI)
RRIDAB_2883649
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式N/A
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

This product is Normal rabbit IgG (without immunized) which purified with Protein A. Normal Rabbit IgG is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody. This antibody can be used as Rabbit IgG control in experiments.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 Rabbit IgG control antibody CL647-30000Download protocol
Standard Protocols
Click here to view our Standard Protocols