Rabbit IgG control Polyclonal antibody

Rabbit IgG control Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

n/a

Applications

WB, IP, ELISA and More (6)

Conjugate

Unconjugated

Cat no : 30000-0-AP

Print datasheet

Synonyms

Rabbit IgG, Rabbit Immunoglobulin, Rabbit IgG isotype, Isotype control, IgG control



经过测试的应用

Positive WB detected inControl
Positive IP detected inControl

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30000-0-AP targets Rabbit IgG control in WB, IHC, IP, CoIP, ChIP, RIP, ELISA, Blocking assay, Cell treatment applications and shows reactivity with n/a samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IP, CoIP, ChIP, RIP, Blocking assay, Cell treatment
Tested Reactivity n/a
Immunogen N/A 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Rabbit IgG control
Synonyms Rabbit IgG, Rabbit Immunoglobulin, Rabbit IgG isotype, Isotype control, IgG control
Gene Symbol
Gene ID (NCBI)
RRIDAB_2819035
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

This product is Normal rabbit IgG (without immunized) which purified with Protein A. Normal Rabbit IgG is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.

发表文章

SpeciesApplicationTitle
IP

Drug Resist Updat

CHD4 promotes acquired chemoresistance and tumor progression by activating the MEK/ERK axis

Authors - Jing Wu
human,mouseWB

Cell Res

Intercellular transfer of activated STING triggered by RAB22A-mediated non-canonical autophagy promotes antitumor immunity

Authors - Ying Gao
IP

Cell Res

RAB31 marks and controls an ESCRT-independent exosome pathway.

Authors - Denghui Wei
IP

ACS Nano

Augmenting Intracellular Cargo Delivery of Extracellular Vesicles in Hypoxic Tissues through Inhibiting Hypoxia-Induced Endocytic Recycling

Authors - Bide Tong
RIP

Nat Commun

ALKBH5-mediated m6A modification of IL-11 drives macrophage-to-myofibroblast transition and pathological cardiac fibrosis in mice

Authors - Tao Zhuang

Nat Commun

EGFR core fucosylation, induced by hepatitis C virus, promotes TRIM40-mediated-RIG-I ubiquitination and suppresses interferon-I antiviral defenses

Authors - Qiu Pan