CoraLite® Plus 488-conjugated Iduronate 2 sulfatase Monoclonal antibody
Iduronate 2 sulfatase Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat , pig
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1A3F9
验证数据展示
经过测试的应用
| Positive IF detected in | HepG2 cells |
For other applications, we recommend the unconjugated version of this antibody, 66112-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66112 targets Iduronate 2 sulfatase in IF applications and shows reactivity with human, mouse, rat , pig samples.
| Tested Applications | IF/ICC |
| Tested Reactivity | human, mouse, rat , pig |
| Immunogen | Iduronate 2 sulfatase fusion protein Ag19095 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | iduronate 2-sulfatase |
| Synonyms | IDS, iduronate 2 sulfatase, Idursulfase, MPS2, SIDS |
| Calculated Molecular Weight | 312aa,35 kDa; 550aa,62 kDa |
| Observed Molecular Weight | 76 kDa, 55 kDa |
| GenBank Accession Number | BC006170 |
| Gene Symbol | IDS |
| Gene ID (NCBI) | 3423 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P22304 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Iduronate 2-sulfatase (IDS) is required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations causing IDS deficiency in humans result in the lysosomal storage of these glycosaminoglycans and Hunter syndrome, an X chromosome-linked disease. IDS is synthesized as two precursor forms of 76 and 90 kDa that are converted, through a 62 kDa intermediate, to 55 and 45 kDa mature polypeptides due to an internal proteolytic cleavage (PMID: 10838181).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Iduronate 2 sulfatase antibody CL488-66112 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |