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验证数据展示

  • WB analysis of human placenta using 66112-1-Ig (same clone as 66112-1-PBS)

    WB analysis of human placenta using 66112-1-Ig (same clone as 66112-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66112-1-Ig (same clone as 66112-1-PBS)

    IHC staining of human liver using 66112-1-Ig (same clone as 66112-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver slide using 66112-1-Ig (Iduronate 2 sulfatase antibody at dilution of 1:50. This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66112-1-Ig (same clone as 66112-1-PBS)

    IHC staining of human liver using 66112-1-Ig (same clone as 66112-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver slide using 66112-1-Ig (Iduronate 2 sulfatase antibody at dilution of 1:50. This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66112-1-Ig (same clone as 66112-1-PBS)

    IF Staining of HepG2 using 66112-1-Ig (same clone as 66112-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Iduronate 2 sulfatase antibody (66112-1-Ig, Clone: 1A3F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

Iduronate 2 sulfatase Monoclonal antibody, PBS Only

Iduronate 2 sulfatase Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66112-1-PBS

产品说明书

CloneNo. 1A3F9

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

IDS, 1A3F9, Alpha-L-iduronate sulfate sulfatase, EC:3.1.6.13, Iduronate 2-sulfatase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66112-1-PBS targets Iduronate 2 sulfatase in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag19095

Product name: Recombinant human IDS protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-312 aa of BC006170

Sequence: MPPPRTGRGLLWLGLVLSSVCVALGSETQANSTTDALNVLLIIVDDLRPSLGCYGDKLVRSPNIDQLASHSLLFQNAFAQQAVCAPSRVSFLTGRRPDTTRLYDFNSYWRVHAGNFSTIPQYFKENGYVTMSVGKVFHPGISSNHTDDSPYSWSFPPYHPSSEKYENTKTCRGPDGELHANLLCPVDVLDVPEGTLPDKQSTEQAIQLLEKMKTSASPFFLAVGYHKPHIPFRYPKEFQKLYPLENITLAPDPEVPDGLPPVAYNPWMDIRQREDVQALNISVPYGPIPVDFQEDQSSTGFRLKTSSTRKYK

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 iduronate 2-sulfatase
别名 IDS, 1A3F9, Alpha-L-iduronate sulfate sulfatase, EC:3.1.6.13, Iduronate 2-sulfatase
计算分子量312aa,35 kDa; 550aa,62 kDa
观测分子量 76 kDa, 55 kDa
GenBank蛋白编号BC006170
基因名称 IDS
Gene ID (NCBI) 3423
RRIDAB_2881511
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP22304
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Iduronate 2-sulfatase (IDS) is required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations causing IDS deficiency in humans result in the lysosomal storage of these glycosaminoglycans and Hunter syndrome, an X chromosome-linked disease. IDS is synthesized as two precursor forms of 76 and 90 kDa that are converted, through a 62 kDa intermediate, to 55 and 45 kDa mature polypeptides due to an internal proteolytic cleavage (PMID: 10838181).