验证数据展示
经过测试的应用
Positive WB detected in | mouse testis tissue |
Positive IF-P detected in | mouse testis tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
13296-1-AP targets IZUMO1 in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IF-P, ELISA Application Description |
经测试反应性 | human, mouse |
免疫原 | IZUMO1 fusion protein Ag4008 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | izumo sperm-egg fusion 1 |
别名 | Sperm-specific protein izumo, OBF, Izumo sperm-egg fusion protein 1, IZUMO |
计算分子量 | 350 aa, 39 kDa |
观测分子量 | 56 kDa |
GenBank蛋白编号 | BC034769 |
基因名称 | IZUMO1 |
Gene ID (NCBI) | 284359 |
RRID | AB_2877934 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q8IYV9 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IZUMO1, also named as OBF, is a sperm-specific protein. It is a novel member of the immunoglobulin superfamily located on sperm and is indispensable for sperm-egg fusion. For mouse Izumo1, the MW is about 56 kDa. For human IZUMO1, the MW is about 37-40 kDa in WB detection.
实验方案
Product Specific Protocols | |
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WB protocol for IZUMO1 antibody 13296-1-AP | Download protocol |
IF protocol for IZUMO1 antibody 13296-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |