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验证数据展示

  • WB analysis using 13296-1-AP

    WB analysis using 13296-1-AP

    Mouse testis tissue were subjected to SDS PAGE followed by western blot with 13296-1-AP (IZUMO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse testis using 13296-1-AP

    IF Staining of mouse testis using 13296-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 13296-1-AP (IZUMO1 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IZUMO1 Polyclonal antibody

IZUMO1 Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 13296-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF-P, ELISA

别名

IZUMO, Izumo sperm-egg fusion protein 1, OBF, Sperm-specific protein izumo

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse testis tissue
Positive IF-P detected inmouse testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13296-1-AP targets IZUMO1 in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag4008

Product name: Recombinant human IZUMO1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 31-294 aa of BC034769

Sequence: LALKSLEKDYLPGHLDAKHHKAMMERVENAVKDFQELSLNEDAYMGVVDEATLQKGSWSLLKDLKRITDSDVKGDLFVKELFWMLHLQKETFATYVARFQKEAYCPNKCGVMLQTLIWCKNCKKEVHACRKSYDCGERNVEVPQMEDMILDCELNWHQASEGLTDYSFYRVWGNNTETLVSKGKEATLTKPMVGPEDAGSYRCELGSVNSSPATIINFHVTVLPKMIKEEKPSPNIVTPGEATTESSISLQPLQPEKMLASRLL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 izumo sperm-egg fusion 1
别名 IZUMO, Izumo sperm-egg fusion protein 1, OBF, Sperm-specific protein izumo
计算分子量 350 aa, 39 kDa
观测分子量70 kDa
GenBank蛋白编号BC034769
基因名称 IZUMO1
Gene ID (NCBI) 284359
RRIDAB_2877934
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IYV9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Izumo sperm egg fusion gene (IZUMO1) is a crucial member of the immunoglobulin superfamily and plays a crucial role in spermatogenesis, sperm egg interaction, and fusion. The expression of IZUMO1 mRNA was mainly in the testis and sperm and performed an important part in fertilization. IZUMO1 possess a well-conserved N-glycosylation site in the middle of an Ig loop among species. Research indicates that the glycosylated form of IZUMO1 has a molecular weight of 56 kDa, while its dimeric form has a molecular weight of 70 kDa (PMID: 21057513, PMID: 31146500).

实验方案

Product Specific Protocols
IF protocol for IZUMO1 antibody 13296-1-APDownload protocol
WB protocol for IZUMO1 antibody 13296-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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