验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue |
| Positive IF-P detected in | mouse testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
13296-1-AP targets IZUMO1 in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF-P, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag4008 Product name: Recombinant human IZUMO1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 31-294 aa of BC034769 Sequence: LALKSLEKDYLPGHLDAKHHKAMMERVENAVKDFQELSLNEDAYMGVVDEATLQKGSWSLLKDLKRITDSDVKGDLFVKELFWMLHLQKETFATYVARFQKEAYCPNKCGVMLQTLIWCKNCKKEVHACRKSYDCGERNVEVPQMEDMILDCELNWHQASEGLTDYSFYRVWGNNTETLVSKGKEATLTKPMVGPEDAGSYRCELGSVNSSPATIINFHVTVLPKMIKEEKPSPNIVTPGEATTESSISLQPLQPEKMLASRLL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | izumo sperm-egg fusion 1 |
| 别名 | IZUMO, Izumo sperm-egg fusion protein 1, OBF, Sperm-specific protein izumo |
| 计算分子量 | 350 aa, 39 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC034769 |
| 基因名称 | IZUMO1 |
| Gene ID (NCBI) | 284359 |
| RRID | AB_2877934 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8IYV9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The Izumo sperm egg fusion gene (IZUMO1) is a crucial member of the immunoglobulin superfamily and plays a crucial role in spermatogenesis, sperm egg interaction, and fusion. The expression of IZUMO1 mRNA was mainly in the testis and sperm and performed an important part in fertilization. IZUMO1 possess a well-conserved N-glycosylation site in the middle of an Ig loop among species. Research indicates that the glycosylated form of IZUMO1 has a molecular weight of 56 kDa, while its dimeric form has a molecular weight of 70 kDa (PMID: 21057513, PMID: 31146500).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for IZUMO1 antibody 13296-1-AP | Download protocol |
| WB protocol for IZUMO1 antibody 13296-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
