验证数据展示
经过测试的应用
| Positive WB detected in | mouse lung tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 1 publications below |
产品信息
14407-1-AP targets ITM2A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | ITM2A fusion protein Ag5692 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | integral membrane protein 2A |
| 别名 | BRICD2A, E25A, integral membrane protein 2A, ITM2A, Protein E25 |
| 计算分子量 | 30 kDa |
| 观测分子量 | 30 kDa |
| GenBank蛋白编号 | BC040437 |
| 基因名称 | ITM2A |
| Gene ID (NCBI) | 9452 |
| RRID | AB_2129683 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O43736 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Integral membrane protein 2A (ITM2A) is a single-pass type II membrane protein belonging to a family,which is composed of at least two other members ITM2B and ITM2C. ITM2A can be induced during thymocyte selection and T cell activation. In early stages of differentiation ITM2A may be associated with an inhibition of the initiation of chondrogenesis.Catalog#14407-1-AP recognises two band of 30 kDa and 45-48 kDa, and the additional 45-48 kDa band may be due to glycosylation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ITM2A antibody 14407-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adipocyte Itm2a silencing rescues lamin A mediated inhibition of 3T3-L1 adipocyte differentiation.
| ||
Front Immunol Integrating machine learning, bioinformatics and experimental verification to identify a novel prognostic marker associated with tumor immune microenvironment in head and neck squamous carcinoma |