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验证数据展示

  • WB analysis of mouse colon using 11770-1-AP (same clone as 11770-1-PBS)

    WB analysis of mouse colon using 11770-1-AP (same clone as 11770-1-PBS)

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 11770-1-AP (ITLN1/2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11770-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse appendix using 11770-1-AP (same clone as 11770-1-PBS)

    IP experiment of mouse appendix using 11770-1-AP (same clone as 11770-1-PBS)

    IP result of anti-ITLN1/2 (IP:11770-1-AP, 4ug; Detection:11770-1-AP 1:500) with mouse appendix tissue lysate 4000ug. This data was developed using the same antibody clone with 11770-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach using 11770-1-AP (same clone as 11770-1-PBS)

    IHC staining of human stomach using 11770-1-AP (same clone as 11770-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11770-1-AP (ITLN1/2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11770-1-PBS in a different storage buffer formulation.

ITLN1/2 Polyclonal antibody, PBS Only

ITLN1/2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 11770-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

ITLN1, HL 1, hIntL, Galactofuranose-binding lectin, Galactofuranose binding lectin

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

11770-1-PBS targets ITLN1/2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2424

Product name: Recombinant human ITLN1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-313 aa of BC020664

Sequence: MNQLSFLLFLIATTRGWSTDEANTYFKEWTCSSSPSLPRSCKEIKDECPSAFDGLYFLRTENGVIYQTFCDMTSGGGGWTLVASVHENDMRGKCTVGDRWSSQQGSKAVYPEGDGNWANYNTFGSAEAATSDDYKNPGYYDIQAKDLGIWHVPNKSPMQHWRNSSLLRYRTDTGFLQTLGHNLFGIYQKYPVKYGEGKCWTDNGPVIPVVYDFGDAQKTASYYSPYGQREFTAGFVQFRVFNNERAANALCAGMRVTGCNTEHHCIGGGGYFPEASPQQCGDFSGFDWSGYGTHVGYSSSREITEAAVLLFYR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 intelectin 1 (galactofuranose binding)
别名 ITLN1, HL 1, hIntL, Galactofuranose-binding lectin, Galactofuranose binding lectin
计算分子量 313 aa, 35 kDa
观测分子量 35-40 kDa
GenBank蛋白编号BC020664
基因名称 ITLN1
Gene ID (NCBI) 55600
RRIDAB_2129677
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WWA0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ITLN1, also named as Intelectin 1, has no effect on basal glucose uptake but enhances insulin-stimulated glucose uptake in adipocytes. ITLN1 is a glycoprotein made up of three 40 kDa subunits cross-linked by disulfide bonds making up a 120-kDa homo-trimer of 295 amino acids and N-linked oligosaccharides (PMID: 17621593). It may play a role in the defense system against microorganisms. Human intelectin-1 is expressed in intestinal goblet cells, and is present at about 200 ng/mL in serum. Intelectin-1 was also shown to be expressed in human endothelial cells and adipocytes of human abdominal adipose tissue. (PMID: 22768319,PMID: 28640441)

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