验证数据展示
经过测试的应用
| Positive WB detected in | mouse colon tissue |
| Positive IP detected in | mouse appendix tissue |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11770-1-AP targets ITLN1/2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag2424 Product name: Recombinant human ITLN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-313 aa of BC020664 Sequence: MNQLSFLLFLIATTRGWSTDEANTYFKEWTCSSSPSLPRSCKEIKDECPSAFDGLYFLRTENGVIYQTFCDMTSGGGGWTLVASVHENDMRGKCTVGDRWSSQQGSKAVYPEGDGNWANYNTFGSAEAATSDDYKNPGYYDIQAKDLGIWHVPNKSPMQHWRNSSLLRYRTDTGFLQTLGHNLFGIYQKYPVKYGEGKCWTDNGPVIPVVYDFGDAQKTASYYSPYGQREFTAGFVQFRVFNNERAANALCAGMRVTGCNTEHHCIGGGGYFPEASPQQCGDFSGFDWSGYGTHVGYSSSREITEAAVLLFYR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | intelectin 1 (galactofuranose binding) |
| 别名 | ITLN1, HL 1, hIntL, Galactofuranose-binding lectin, Galactofuranose binding lectin |
| 计算分子量 | 313 aa, 35 kDa |
| 观测分子量 | 35-40 kDa |
| GenBank蛋白编号 | BC020664 |
| 基因名称 | ITLN1 |
| Gene ID (NCBI) | 55600 |
| RRID | AB_2129677 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8WWA0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ITLN1, also named as Intelectin 1, has no effect on basal glucose uptake but enhances insulin-stimulated glucose uptake in adipocytes. ITLN1 is a glycoprotein made up of three 40 kDa subunits cross-linked by disulfide bonds making up a 120-kDa homo-trimer of 295 amino acids and N-linked oligosaccharides (PMID: 17621593). It may play a role in the defense system against microorganisms. Human intelectin-1 is expressed in intestinal goblet cells, and is present at about 200 ng/mL in serum. Intelectin-1 was also shown to be expressed in human endothelial cells and adipocytes of human abdominal adipose tissue. (PMID: 22768319,PMID: 28640441)
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for ITLN1/2 antibody 11770-1-AP | Download protocol |
| IP protocol for ITLN1/2 antibody 11770-1-AP | Download protocol |
| WB protocol for ITLN1/2 antibody 11770-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Mater Biomimetic Nanomotors for Deep Ischemia Penetration and Ferroptosis Inhibition in Neuroprotective Therapy of Ischemic Stroke | ||
Nat Commun Tissue mechanics drives regeneration of a mucociliated epidermis on the surface of Xenopus embryonic aggregates. | ||
Sci Adv Lectin-Seq: A method to profile lectin-microbe interactions in native communities | ||
Int J Biol Sci ITLN1 exacerbates Crohn's colitis by driving ZBP1-dependent PANoptosis in intestinal epithelial cells through antagonizing TRIM8-mediated CAPN2 ubiquitination |
