验证数据展示
经过测试的应用
| Positive WB detected in | mouse retina tissue, NIH/3T3 cells |
| Positive IHC detected in | mouse eye tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | NIH/3T3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83782-5-RR targets ISLR in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag19446 Product name: Recombinant human ISLR protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 318-402 aa of BC022478 Sequence: GKLEEGTYSCLATNELGSAESSVDVALATPGEGGEDTLGRRFHGKAVEGKGCYTVDNEVQPSGPEDNVVIIYLSRAGNPEAAVAE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | immunoglobulin superfamily containing leucine-rich repeat |
| 别名 | 240727D4, HsT17563, Immunoglobulin superfamily containing leucine-rich repeat protein, UNQ189/PRO215 |
| 计算分子量 | 428 aa, 46 kDa |
| 观测分子量 | 44-46 kDa |
| GenBank蛋白编号 | BC022478 |
| 基因名称 | ISLR |
| Gene ID (NCBI) | 3671 |
| RRID | AB_3671374 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | O14498 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The Immunoglobulin superfamily containing leucine-rich repeat protein(ISLR), is involved in various biological events, such as embryonic development, Gaucher's disease, and replicative senescence of fibroblasts in some organs, including the heart, pancreas, and bone marrow. ISLR, also known as meflin, is a newly discovered marker of mesenchymal stem cells, which is involved in pathological fibrosis and the cancer microenvironment. (PMID: 34713300)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for ISLR antibody 83782-5-RR | Download protocol |
| IHC protocol for ISLR antibody 83782-5-RR | Download protocol |
| WB protocol for ISLR antibody 83782-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
