验证数据展示
经过测试的应用
| Positive WB detected in | Raji cells, Daudi cells |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy ISG20 Ready-To-Use IHC Kit. ISG20 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22097-1-AP targets ISG20 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, canine, monkey |
| 免疫原 |
CatNo: Ag17303 Product name: Recombinant human ISG20 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-150 aa of BC007922 Sequence: MAGSREVVAMDCEMVGLGPHRESGLARCSLVNVHGAVLYDKFIRPEGEITDYRTRVSGVTPQHMVGATPFAVARLEILQLLKGKLVVGHDLKHDFQALKEDMSGYTIYDTSTDRLLWREAKLDHCRRVSLRVLSERLLHKSIQNSLLGHS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | interferon stimulated exonuclease gene 20kDa |
| 别名 | EC:3.1.13.1, Estrogen-regulated transcript 45 protein, HEM45, Interferon-stimulated gene 20 kDa protein, Promyelocytic leukemia nuclear body-associated protein ISG20 |
| 计算分子量 | 181 aa, 20 kDa |
| 观测分子量 | 20 kDa |
| GenBank蛋白编号 | BC007922 |
| 基因名称 | ISG20 |
| Gene ID (NCBI) | 3669 |
| RRID | AB_2878994 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96AZ6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for ISG20 antibody 22097-1-AP | Download protocol |
| WB protocol for ISG20 antibody 22097-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Redox Biol Activation of sclerostin inhibits Isg20-Mediated aerobic glycolysis ameliorating renal Fibrosis: the renoprotective mechanism of hederagenin in CKD | ||
Mol Cell RNA exonuclease REXO4 resolves m(6)A-marked R-loops and suppresses anti-tumor immunity. | ||
Nucleic Acids Res Internal RNA 2'O-methylation in the HIV-1 genome counteracts ISG20 nuclease-mediated antiviral effect
| ||
Cell Rep Enzymolysis-based RNA pull-down identifies YTHDC2 as an inhibitor of antiviral innate response | ||
Cancer Cell Int Identification and validation of a novel glycolysis-related gene signature for predicting the prognosis in ovarian cancer. |
