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IRF6 Polyclonal antibody

IRF6 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 12928-1-AP

Print datasheet

Synonyms

interferon regulatory factor 6, IRF 6, IRF6, LPS, OFC6, PIT, PPS, VWS

验证数据展示

  • IHC staining of human lung using 12928-1-AP

    IHC staining of human lung using 12928-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 12928-1-AP (IRF6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IRF6 antibody included
  • WB analysis using 12928-1-AP

    WB analysis using 12928-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12928-1-AP (IRF6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung using 12928-1-AP

    IHC staining of human lung using 12928-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 12928-1-AP (IRF6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IRF6 antibody included

经过测试的应用

Positive WB detected inA431 cells, A549 cells, HaCaT cells
Positive IHC detected inhuman lung tissue, mouse kidney tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy IRF6 Ready-To-Use IHC Kit. IRF6 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12928-1-AP targets IRF6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen IRF6 fusion protein Ag3623 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name interferon regulatory factor 6
Synonyms interferon regulatory factor 6, IRF 6, IRF6, LPS, OFC6, PIT, PPS, VWS
Calculated Molecular Weight 467 aa, 53 kDa
Observed Molecular Weight53-60 kDa
GenBank Accession NumberBC014852
Gene Symbol IRF6
Gene ID (NCBI) 3664
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14896
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The interferon regulatory factor (IRF) family of genes directs the expression and activity of interferon and thus can strictly regulate innate immunity. Interferon regulatory factor 6 (IRF6) belongs to a family of nine transcription factors that share a highly conserved helix−turn−helix DNA-binding domain and a less conserved protein-binding domain. IRF6 regulates the balance between immature ectoderm differentiation and proliferation during craniofacial morphogenesis. IRF6 functions as a tumor suppressor gene in breast cancer, cervical cancer, nasopharyngeal carcinoma and so on.

实验方案

Product Specific Protocols
WB protocol for IRF6 antibody 12928-1-APDownload protocol
IHC protocol for IRF6 antibody 12928-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols