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验证数据展示

  • WB analysis using 10547-1-AP (same clone as 10547-1-PBS)

    WB analysis using 10547-1-AP (same clone as 10547-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 10547-1-AP (IRF5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10547-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 10547-1-AP (same clone as 10547-1-PBS)

    IF Staining of HepG2 using 10547-1-AP (same clone as 10547-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IRF5 antibody (10547-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10547-1-PBS in a different storage buffer formulation.

  • FC experiment of THP-1 using 10547-1-AP (same clone as 10547-1-PBS)

    FC experiment of THP-1 using 10547-1-AP (same clone as 10547-1-PBS)

    1x10^6 THP-1 cells were intracellularly stained with 0.25 ug IRF5 Polyclonal antibody (10547-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 10547-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 10547-1-AP (same clone as 10547-1-PBS)

    IHC staining of human prostate cancer using 10547-1-AP (same clone as 10547-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10547-1-AP (IRF5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10547-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 10547-1-AP (same clone as 10547-1-PBS)

    IP experiment of HeLa using 10547-1-AP (same clone as 10547-1-PBS)

    IP result of anti-IRF5 (IP:10547-1-AP, 4ug; Detection:10547-1-AP 1:300) with HeLa cells lysate 1600ug. This data was developed using the same antibody clone with 10547-1-PBS in a different storage buffer formulation.

IRF5 Polyclonal antibody, PBS Only

IRF5 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 10547-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

别名

interferon regulatory factor 5, IRF 5, IRF-5 , SLEB10

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

10547-1-PBS targets IRF5 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0841

Product name: Recombinant human IRF5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 173-498 aa of BC004201

Sequence: TLRPPTLQPPTLQPPVVLGPPAPDPSPLAPPPGNPAGFRELLSEVLEPGPLPASLPPAGEQLLPDLLISPHMLPLTDLEIKFQYRGRPPRALTISNPHGCRLFYSQLEATQEQVELFGPISLEQVRFPSPEDIPSDKQRFYTNQLLDVLDRGLILQLQGQDLYAIRLCQCKVFWSGPCASAHDSCPNPIQREVKTKLFSLEHFLNELILFQKGQTNTPPPFEIFFCFGEEWPDRKPREKKLITVQVVPVAARLLLEMFSGELSWSADSIRLQISNPDLKDRMVEQFKELHHIWQSQQRLQPVAQAPPGAGLGVGQGPWPMHPAGMQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 interferon regulatory factor 5
别名 interferon regulatory factor 5, IRF 5, IRF-5 , SLEB10
计算分子量 56 kDa
观测分子量 56 kDa
GenBank蛋白编号BC004201
基因名称 IRF5
Gene ID (NCBI) 3663
RRIDAB_2280474
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13568
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IRF5, also named as SLEB10, contians one IRF tryptophan pentad repeat DNA-binding domain and belongs to the IRF family. It is a transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. It is activated by TLR7 or TLR8 signaling. Genetic variations in IRF5 are associated with susceptibility to inflammatory bowel disease type 14 (IBD14) and systemic lupus erythematosus type 10 (SLEB10). Alternative splice variant encoding different isoforms exist. Catalog#10547-1-AP is a rabbit polyclonal antibody raised against the C-terminal of human IRF5.

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