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验证数据展示

  • WB analysis using 66451-1-Ig (same clone as 66451-1-PBS)

    WB analysis using 66451-1-Ig (same clone as 66451-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66451-1-Ig (IRF4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66451-1-PBS in a different storage buffer formulation.

  • WB analysis using 66451-1-Ig (same clone as 66451-1-PBS)

    WB analysis using 66451-1-Ig (same clone as 66451-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66451-1-Ig (IRF4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66451-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66451-1-Ig (same clone as 66451-1-PBS)

    IHC staining of human tonsillitis using 66451-1-Ig (same clone as 66451-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66451-1-Ig (IRF4 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66451-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IRF4 antibody included
  • IHC staining of human tonsillitis using 66451-1-Ig (same clone as 66451-1-PBS)

    IHC staining of human tonsillitis using 66451-1-Ig (same clone as 66451-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66451-1-Ig (IRF4 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66451-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IRF4 antibody included

IRF4 Monoclonal antibody, PBS Only

IRF4 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66451-1-PBS

产品说明书

CloneNo. 1A12D5

宿主/亚型

Mouse / IgG1

种属反应性

human, rat, pig

应用

WB, IHC, Indirect ELISA

别名

MUM1/IRF4, 1A12D5, interferon regulatory factor 4, IRF 4, IRF-4

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy IRF4 Ready-To-Use IHC Kit. IRF4 primary antibody included.

产品信息

66451-1-PBS targets IRF4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat, pig samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, rat, pig
免疫原

CatNo: Ag25203

Product name: Recombinant human IRF4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-451 aa of BC015752

Sequence: MNLEGGGRGGEFGMSAVSCGNGKLRQWLIDQIDSGKYPGLVWENEEKSIFRIPWKHAGKQDYNREEDAALFKAWALFKGKFREGIDKPDPPTWKTRLRCALNKSNDFEELVERSQLDISDPYKVYRIVPEGAKKGAKQLTLEDPQMSMSHPYTMTTPYPSLPAQQVHNYMMPPLDRSWRDYVPDQPHPEIPYQCPMTFGPRGHHWQGPACENGCQVTGTFYACAPPESQAPGVPTEPSIRSAEALAFSDCRLHICLYYREILVKELTTSSPEGCRISHGHTYDASNLDQVLFPYPEDNGQRKNIEKLLSHLERGVVLWMAPDGLYAKRLCQSRIYWDGPLALCNDRPNKLERDQTCKLFDTQQFLSELQAFAHHGRSLPRFQVTLCFGEEFPDPQRQRKLITAHVEPLLARQLYYFAQQNSGHFLRGYDLPEHISNPEDYHRSIRHSSIQE

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 IRF 4
别名 MUM1/IRF4, 1A12D5, interferon regulatory factor 4, IRF 4, IRF-4
计算分子量 52 kDa
观测分子量 48-52 kDa
GenBank蛋白编号BC015752
基因名称 IRF4
Gene ID (NCBI) 3662
ENSEMBL Gene IDENSG00000137265
RRIDAB_2881820
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ15306
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IF regulatory factor 4 (IRF4) is a member of the IRF family of transcription factors, expressed in most cell types of the immune system. IRF4 is a transcription factor essential for the development of T helper-2 (Th2) cells, IL17-producing Th17 cells, and IL9-producing Th9 cells, as well as dendritic cell (DC). It binds to and activates the IF-stimulated response element (ISRE) of the MHC class I promoter, and may have a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells.

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