验证数据展示
经过测试的应用
| Positive WB detected in | U266 cells, human spleen tissue, pig spleen tissue, rat spleen tissue, HuT 78 cells, MJ cells |
| Positive IHC detected in | human tonsillitis Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66451-1-Ig targets IRF4 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, rat, pig samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, CoIP |
| 经测试反应性 | human, rat, pig |
| 文献引用反应性 | human, mouse, pig |
| 免疫原 |
CatNo: Ag25203 Product name: Recombinant human IRF4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-451 aa of BC015752 Sequence: MNLEGGGRGGEFGMSAVSCGNGKLRQWLIDQIDSGKYPGLVWENEEKSIFRIPWKHAGKQDYNREEDAALFKAWALFKGKFREGIDKPDPPTWKTRLRCALNKSNDFEELVERSQLDISDPYKVYRIVPEGAKKGAKQLTLEDPQMSMSHPYTMTTPYPSLPAQQVHNYMMPPLDRSWRDYVPDQPHPEIPYQCPMTFGPRGHHWQGPACENGCQVTGTFYACAPPESQAPGVPTEPSIRSAEALAFSDCRLHICLYYREILVKELTTSSPEGCRISHGHTYDASNLDQVLFPYPEDNGQRKNIEKLLSHLERGVVLWMAPDGLYAKRLCQSRIYWDGPLALCNDRPNKLERDQTCKLFDTQQFLSELQAFAHHGRSLPRFQVTLCFGEEFPDPQRQRKLITAHVEPLLARQLYYFAQQNSGHFLRGYDLPEHISNPEDYHRSIRHSSIQE 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | IRF 4 |
| 别名 | MUM1/IRF4, 1A12D5, interferon regulatory factor 4, IRF 4, IRF-4 |
| 计算分子量 | 52 kDa |
| 观测分子量 | 48-52 kDa |
| GenBank蛋白编号 | BC015752 |
| 基因名称 | IRF4 |
| Gene ID (NCBI) | 3662 |
| ENSEMBL Gene ID | ENSG00000137265 |
| RRID | AB_2881820 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q15306 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IF regulatory factor 4 (IRF4) is a member of the IRF family of transcription factors, expressed in most cell types of the immune system. IRF4 is a transcription factor essential for the development of T helper-2 (Th2) cells, IL17-producing Th17 cells, and IL9-producing Th9 cells, as well as dendritic cell (DC). It binds to and activates the IF-stimulated response element (ISRE) of the MHC class I promoter, and may have a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for IRF4 antibody 66451-1-Ig | Download protocol |
| WB protocol for IRF4 antibody 66451-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Bioeng Transl Med Macrophage-targeted delivery of siRNA to silence Mecp2 gene expression attenuates pulmonary fibrosis. | ||
Biochim Biophys Acta Mol Cell Res RNF2 promotes the progression of colon cancer by regulating ubiquitination and degradation of IRF4 | ||
FEBS J TBK1 is involved in M-CSF-induced macrophage polarization through mediating the IRF5/IRF4 axis | ||
Adv Sci (Weinh) Mechanisms of Baicalin Alleviates Intestinal Inflammation: Role of M1 Macrophage Polarization and Lactobacillus amylovorus |
