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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated IRF1 Polyclonal antibody

IRF1 Polyclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-11335

Print datasheet

Synonyms

interferon regulatory factor 1, IRF 1, IRF1, MAR

验证数据展示

  • IF Staining of HepG2 using CL488-11335

    IF Staining of HepG2 using CL488-11335

    Immunofluorescent analysis of (-20°C Ethanol) fixed IFN gamma treated HepG2 cells using CoraLite® Plus 488 IRF1 antibody (CL488-11335) at dilution of 1:200.

  • FC experiment of Jurkat using CL488-11335

    FC experiment of Jurkat using CL488-11335

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human IRF1 (CL488-11335) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF detected inIFN gamma treated HepG2 cells
Positive FC detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 11335-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11335 targets IRF1 in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen IRF1 fusion protein Ag1879 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name IRF 1
Synonyms interferon regulatory factor 1, IRF 1, IRF1, MAR
Calculated Molecular Weight 325 aa, 37 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC009483
Gene Symbol IRF1
Gene ID (NCBI) 3659
RRIDAB_2919026
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP10914
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IRF1, also named as IFN regulatory factor 1, is a 325 amino acid protein, which belongs to the IRF family. IRF1 as a transcriptional regulator which displays a remarkable functional diversity in the regulation of cellular responses. The calculated molecular weight of IRF1 is 37 kDa, but the modified IRF1 protein is about 45-50 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 IRF1 antibody CL488-11335Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human

Sci Adv

XAF1 promotes anti-RNA virus immune responses by regulating chromatin accessibility

Authors - Ming Kuang
View Article