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验证数据展示

  • WB analysis using 29976-1-AP (same clone as 29976-1-PBS)

    WB analysis using 29976-1-AP (same clone as 29976-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29976-1-AP (IREB2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29976-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse liver using 29976-1-AP (same clone as 29976-1-PBS)

    IP experiment of mouse liver using 29976-1-AP (same clone as 29976-1-PBS)

    IP result of anti-IREB2 (IP:29976-1-AP, 4ug; Detection:29976-1-AP 1:5000) with mouse liver tissue lysate 1840 ug. This data was developed using the same antibody clone with 29976-1-PBS in a different storage buffer formulation.

  • IP experiment of A549 using 29976-1-AP (same clone as 29976-1-PBS)

    IP experiment of A549 using 29976-1-AP (same clone as 29976-1-PBS)

    IP result of anti-IREB2 (IP:29976-1-AP, 4ug; Detection:29976-1-AP 1:1000) with A549 cells lysate 920 ug. This data was developed using the same antibody clone with 29976-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 29976-1-AP (same clone as 29976-1-PBS)

    IHC staining of human colon cancer using 29976-1-AP (same clone as 29976-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29976-1-AP (IREB2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29976-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IREB2 antibody included
  • IHC staining of human colon cancer using 29976-1-AP (same clone as 29976-1-PBS)

    IHC staining of human colon cancer using 29976-1-AP (same clone as 29976-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29976-1-AP (IREB2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29976-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IREB2 antibody included
  • IF Staining of A549 using 29976-1-AP (same clone as 29976-1-PBS)

    IF Staining of A549 using 29976-1-AP (same clone as 29976-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using IREB2 antibody (29976-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29976-1-PBS in a different storage buffer formulation.

IREB2 Polyclonal antibody, PBS Only

IREB2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 29976-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy IREB2 Ready-To-Use IHC Kit. IREB2 primary antibody included.

产品信息

29976-1-PBS targets IREB2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag32233

Product name: Recombinant human IREB2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 50-255 aa of BC017880

Sequence: VRNCDGFLMKKEDVMNILDWKTKQSNVEVPFFPARVLLQDFTGIPAMVDFAAMREAVKTLGGDPEKVHPACPTDLTVDHSLQIDFSKCAIQNAPNPGGGDLQKAGKLSPLKVQPKKLPCRGQTTCRGSCDSGELGRNSGTFSSQIENTPILCPFHLQPVPEPETVLKNQEVEFGRNRERLQFFKWSSRVFKNVAVIPPGTGMAHQI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 iron-responsive element binding protein 2
别名
计算分子量 105 kDa
观测分子量105 kDa
GenBank蛋白编号BC017880
基因名称 IREB2
Gene ID (NCBI) 3658
RRIDAB_3086201
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP48200
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IREB2 is an RNA-binding protein that acts to regulate iron levels in the cells by regulating the translation and stability of mRNAs that affect iron homeostasis under conditions when iron is depleted. When iron levels are low, this protein binds to iron-responsive elements (IRES), stem-loop structures located either in the 5' or 3' UTRs. Binding to the 5' UTR represses translation, while binding to the 3' UTR inhibits mRNA degradation. When iron is found in the cell, this protein is degraded in a F-box and leucine rich repeat protein 5-dependent manner.

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