• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

IREB2 Polyclonal antibody

IREB2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 29976-1-AP

Print datasheet

Synonyms

ACO3, FLJ23381, IRE BP 2, IREB2, Iron regulatory protein 2, IRP2, IRP2AD

验证数据展示

  • WB analysis using 29976-1-AP

    WB analysis using 29976-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29976-1-AP (IREB2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse liver using 29976-1-AP

    IP experiment of mouse liver using 29976-1-AP

    IP result of anti-IREB2 (IP:29976-1-AP, 4ug; Detection:29976-1-AP 1:5000) with mouse liver tissue lysate 1840 ug.

  • IP experiment of A549 using 29976-1-AP

    IP experiment of A549 using 29976-1-AP

    IP result of anti-IREB2 (IP:29976-1-AP, 4ug; Detection:29976-1-AP 1:1000) with A549 cells lysate 920 ug.

  • IHC staining of human colon cancer using 29976-1-AP

    IHC staining of human colon cancer using 29976-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29976-1-AP (IREB2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 29976-1-AP

    IHC staining of human colon cancer using 29976-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29976-1-AP (IREB2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse liver tissue
Positive IP detected inA549 cells, mouse liver tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29976-1-AP targets IREB2 in WB, IP, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications IHC, IP, WB, ELISA
Tested Reactivity Human, mouse
Immunogen IREB2 fusion protein Ag32233 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name iron-responsive element binding protein 2
Synonyms ACO3, FLJ23381, IRE BP 2, IREB2, Iron regulatory protein 2, IRP2, IRP2AD
Calculated Molecular Weight 105 kDa
Observed Molecular Weight105 kDa
GenBank Accession NumberBC017880
Gene Symbol IREB2
Gene ID (NCBI) 3658
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP48200
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IREB2 is an RNA-binding protein that acts to regulate iron levels in the cells by regulating the translation and stability of mRNAs that affect iron homeostasis under conditions when iron is depleted. When iron levels are low, this protein binds to iron-responsive elements (IRES), stem-loop structures located either in the 5' or 3' UTRs. Binding to the 5' UTR represses translation, while binding to the 3' UTR inhibits mRNA degradation. When iron is found in the cell, this protein is degraded in a F-box and leucine rich repeat protein 5-dependent manner.

实验方案

Product Specific Protocols
WB protocol for IREB2 antibody 29976-1-APDownload protocol
IHC protocol for IREB2 antibody 29976-1-APDownload protocol
IP protocol for IREB2 antibody 29976-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols