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验证数据展示

  • WB analysis of HEK-293 using 66653-1-Ig (same clone as 66653-1-PBS)

    WB analysis of HEK-293 using 66653-1-Ig (same clone as 66653-1-PBS)

    WB result of IRAK1 antibody (66653-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IRAK1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66653-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66653-1-Ig (same clone as 66653-1-PBS)

    WB analysis of HeLa using 66653-1-Ig (same clone as 66653-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66653-1-Ig (IRAK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66653-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66653-1-Ig (same clone as 66653-1-PBS)

    IHC staining of human lung cancer using 66653-1-Ig (same clone as 66653-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66653-1-Ig (IRAK1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66653-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IRAK1 antibody included
  • IHC staining of human lung cancer using 66653-1-Ig (same clone as 66653-1-PBS)

    IHC staining of human lung cancer using 66653-1-Ig (same clone as 66653-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66653-1-Ig (IRAK1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66653-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IRAK1 antibody included
  • Featured Product
  • KD/KO验证

IRAK1 Monoclonal antibody, PBS Only

IRAK1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66653-1-PBS

产品说明书

CloneNo. 1H10A7

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

WB, IHC, Indirect ELISA

别名

IRAK, IRAK 1, IRAK1, pelle

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy IRAK1 Ready-To-Use IHC Kit. IRAK1 primary antibody included.

产品信息

66653-1-PBS targets IRAK1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag0728

Product name: Recombinant human IRAK1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 420-633 aa of BC014963

Sequence: GQRAVKTHGARTKYLVYERLEKLQAVVAGVPGHLEAASCIPPSPQENSYVSSTGRAHSGAAPWQPLAAPSGASAQAAEQLQRGPNQPVESDESLGGLSAALRSWHLTPSCPLDPAPLREAGCPQGDTAGESSWGSGPGSRPTAVEGLALGSSASSSSEPPQIIINPARQKMVQKLALYEDGALDSLQLLSSSSLPGLGLEQDRQGPEESDEFQS

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 interleukin-1 receptor-associated kinase 1
别名 IRAK, IRAK 1, IRAK1, pelle
计算分子量 77 kDa
观测分子量 80 kDa
GenBank蛋白编号BC014963
基因名称 IRAK1
Gene ID (NCBI) 3654
RRIDAB_2882010
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP51617
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Interleukin-1 receptor-associated kinases (IRAKs) are a unique family of death domain containing protein kinases that play a key role in initiating innate immune response against foreign pathogens. They are involved in Toll-like receptor (TLR) and interleukin-1 receptor (IL-1R) signaling pathways. IRAK1 is the first member of this kinase family. Upon ligand binding to TLR/IL-1R, IRAK1 is recruited by MYD88 to the receptor-signaling complex, the association leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Hyper-phosphorylated IRAK1 then disengages from the receptor complex, and forms a cytosolic IRAK1-TRAF6 complex. TRAF6 then interacts with TAK and TAB, resulting in eventual activation of the NF-κB and MAPK pathways. Phosphorylated IRAK1 also undergoes ubiquitin-mediated degradation or sumoylation, which results in nuclear translocation and transcriptional activation of inflammatory target genes. (PMID: 17890055; 12620219)

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