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验证数据展示

  • WB analysis of MCF-7 using 84610-3-RR

    WB analysis of MCF-7 using 84610-3-RR

    WB result of INTS6 antibody (84610-3-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-INTS6 transfected MCF-7 cells.

  • IHC staining of mouse liver using 84610-3-RR

    IHC staining of mouse liver using 84610-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84610-3-RR (INTS6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 84610-3-RR

    IHC staining of mouse liver using 84610-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84610-3-RR (INTS6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 84610-3-RR

    IF Staining of HeLa using 84610-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using INTS6 antibody (84610-3-RR, Clone: 242099E6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of U-251 using 84610-3-RR

    FC experiment of U-251 using 84610-3-RR

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug INTS6 Recombinant antibody (84610-3-RR, Clone:242099E6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 84610-3-RR

    Affinity and Kinetic Characterization of 84610-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84610-3-RR against Human INTS6 were performed. The affinity constant is 1.71 nM.

  • Featured Product
  • KD/KO验证

INTS6 Recombinant monoclonal antibody

INTS6 Uni-rAb® Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 84610-3-RR

产品说明书

CloneNo. 242099E6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, FC (Intra), ELISA

别名

242099E6, DBI 1, DBI1, DDX26, DDX26A

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inU-251 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84610-3-RR targets INTS6 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag36146

Product name: Recombinant human INTS6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 672-815 aa of BC039829

Sequence: VVNNHIGGKGPPAPTTQAQPDLIKPLPLHKISETTNDSIIHDVVENHVADQLSSDITPNAMDTEFSASSPASLLERPTNHMEALGHDHLGTNDLTVGGFLENHEEPRDKEQCAEENIPASSLNKGKKLMHCRSHEEVNTELKAQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 integrator complex subunit 6
别名 242099E6, DBI 1, DBI1, DDX26, DDX26A
计算分子量 100 kDa
GenBank蛋白编号BC039829
基因名称 INTS6
Gene ID (NCBI) 26512
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9UL03
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

INTS6 is a component of the Integrator (INT) complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing.

实验方案

Product Specific Protocols
FC protocol for INTS6 antibody 84610-3-RRDownload protocol
IF protocol for INTS6 antibody 84610-3-RRDownload protocol
IHC protocol for INTS6 antibody 84610-3-RRDownload protocol
WB protocol for INTS6 antibody 84610-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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