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验证数据展示

  • WB analysis using 84610-1-RR (same clone as 84610-1-PBS)

    WB analysis using 84610-1-RR (same clone as 84610-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84610-1-RR (INTS6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84610-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01446-1

    Sandwich ELISA standard curve of MP01446-1

    Sandwich ELISA standard curve of MP01446-1, Human INTS6 Recombinant Matched Antibody Pair - PBS only. 84610-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag36146. 84610-1-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

  • Sample test of MP01446-1

    Sample test of MP01446-1

    The mean INTS6 concentration was determined to be 61.44 ng/mL in DU 145 cell extract based on a 1.00 mg/mL extract load.

  • IF Staining of HeLa using 84610-1-RR (same clone as 84610-1-PBS)

    IF Staining of HeLa using 84610-1-RR (same clone as 84610-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using INTS6 antibody (84610-1-RR, Clone: 242099C7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84610-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84610-1-RR (same clone as 84610-1-PBS)

    IF Staining of HeLa using 84610-1-RR (same clone as 84610-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using INTS6 antibody (84610-1-RR, Clone: 242099C7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84610-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01446-1

    Cytometric bead array standard curve of MP01446-1

    Cytometric bead array standard curve of MP01446-1, INTS6 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84610-3-PBS. Detection antibody: 84610-1-PBS. Standard: Ag36146. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 84610-1-PBS

    Affinity and Kinetic Characterization of 84610-1-PBS

    Biolayer interferometry (BLl) kinetic assays of 84610-1-PBS against Human INTS6 were performed. The affinity constant is 0.63 nM.

  • Affinity and Kinetic Characterization of 84610-1-RR

    Affinity and Kinetic Characterization of 84610-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84610-1-RR against Human INTS6 were performed. The affinity constant is 0.63 nM.

INTS6 Recombinant monoclonal antibody, PBS Only (Detector)

INTS6 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84610-1-PBS

产品说明书

CloneNo. 242099C7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

242099C7, DBI 1, DBI1, DDX26, DDX26A

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84610-1-PBS targets INTS6 as part of a matched antibody pair:

MP01446-1: 84610-3-PBS capture and 84610-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原

CatNo: Ag36146

Product name: Recombinant human INTS6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 672-815 aa of BC039829

Sequence: VVNNHIGGKGPPAPTTQAQPDLIKPLPLHKISETTNDSIIHDVVENHVADQLSSDITPNAMDTEFSASSPASLLERPTNHMEALGHDHLGTNDLTVGGFLENHEEPRDKEQCAEENIPASSLNKGKKLMHCRSHEEVNTELKAQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 integrator complex subunit 6
别名 242099C7, DBI 1, DBI1, DDX26, DDX26A
计算分子量 100 kDa
观测分子量130 kDa
GenBank蛋白编号BC039829
基因名称 INTS6
Gene ID (NCBI) 26512
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9UL03
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

INTS6 is a component of the Integrator (INT) complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing.

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