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验证数据展示

  • IF Staining of U2OS using CL488-83154

    IF Staining of U2OS using CL488-83154

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 INTS5 antibody (CL488-83154, Clone: 230462B10 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-83154

    FC experiment of HeLa using CL488-83154

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Ints5 Recombinant Antibody (CL488-83154, Clone:230462B10)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

CoraLite® Plus 488-conjugated INTS5 Recombinant monoclonal antibody

INTS5 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra)
Cat No. CL488-83154

产品说明书

CloneNo. 230462B10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC, FC (Intra)

别名

INTS 5, Int5, 230462B10

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy INTS5 Ready-To-Use IHC Kit. INTS5 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83154-1-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83154 targets INTS5 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag5129

Product name: Recombinant human INTS5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-322 aa of BC060841

Sequence: MSALCDPPGAPGPPGPAPATHGPAPLSAQELSQEIKAFLTGVDPILGHQLSAREHARCGLLLLRSLPPARAAVLDHLRGVFDESVRAHLAALDETPVAGPPHLRPPPPSHVPAGGPGLEDVVQEVQQVLSEFIRANPKAWAPVISAWSIDLMGQLSSTYSGQHQRVPHATGALNELLQLWMGCRATRTLMDIYVQCLSALIGSCPDACVDALLDTSVQHSPHFDWVVAHIGSSFPGTIISRVLSCGLKDFCVHGGAGGGAGSSGGSSSQTPSTDPFPGSPAIPAEKRVPKIASVVGILGHLASRHGDSIRRELLRMFHDSLA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 integrator complex subunit 5
别名 INTS 5, Int5, 230462B10
计算分子量 108 kDa
观测分子量108 kDa
GenBank蛋白编号BC060841
基因名称 INTS5
Gene ID (NCBI) 80789
RRIDAB_3673204
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ6P9B9
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

INTS5, also named as INT5 and KIAA1698, is a component of the Integrator complex which involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 INTS5 antibody CL488-83154Download protocol
Standard Protocols
Click here to view our Standard Protocols
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