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验证数据展示

  • WB analysis using 17997-1-AP (same clone as 17997-1-PBS)

    WB analysis using 17997-1-AP (same clone as 17997-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 17997-1-AP (INSL3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17997-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 17997-1-AP

    IHC staining of human testis using 17997-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17997-1-AP (INSL3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human testis using 17997-1-AP

    IHC staining of human testis using 17997-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17997-1-AP (INSL3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

INSL3 Polyclonal antibody, PBS Only

INSL3 Polyclonal Antibody for WB, ELISA
Cat No. 17997-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

Insulin like 3, Insulin-like 3, Insulin-like 3 A chain, Insulin-like 3 B chain, Leydig insulin-like peptide

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

17997-1-PBS targets INSL3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12455

Product name: Recombinant human INSL3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 17-131 aa of BC071706

Sequence: LVFALGPAPTPEMREKLCGHHFVRALVRVCGGPRWSTEARRPAAGGDRELLQWLERRHLLHGLVADSNLTLGPGLQPLPQTSHHHRHHRAAATNPARYCCLSGCTQQDLLTLCPY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 insulin-like 3 (Leydig cell)
别名 Insulin like 3, Insulin-like 3, Insulin-like 3 A chain, Insulin-like 3 B chain, Leydig insulin-like peptide
计算分子量 131 aa, 14 kDa
观测分子量18 kDa
GenBank蛋白编号BC071706
基因名称 INSL3
Gene ID (NCBI) 3640
RRIDAB_2878479
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP51460
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Insulin-like 3 (INSL3), a Leydig cell-specific hormone, is essential for testis descent during foetal life and bone metabolism in adults. Insl3-deficient mice exhibit bilateral undescended testes located high in the abdominal cavity. INSL3 is presumed also to conform to a heterodimeric A-B structure of approximately 6 kDa once released from the producing cells. It is possible that both the mature form of INSL3 (6.6 kDa) and the pro-form(s) of INSL3 (12-14 kDa) are secreted from mammalian Leydig cells (PMID: 19329805).

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