验证数据展示
经过测试的应用
| Positive WB detected in | human testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
17997-1-AP targets INSL3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | rat |
| 免疫原 |
CatNo: Ag12455 Product name: Recombinant human INSL3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 17-131 aa of BC071706 Sequence: LVFALGPAPTPEMREKLCGHHFVRALVRVCGGPRWSTEARRPAAGGDRELLQWLERRHLLHGLVADSNLTLGPGLQPLPQTSHHHRHHRAAATNPARYCCLSGCTQQDLLTLCPY 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | insulin-like 3 (Leydig cell) |
| 别名 | Insulin like 3, Insulin-like 3, Insulin-like 3 A chain, Insulin-like 3 B chain, Leydig insulin-like peptide |
| 计算分子量 | 131 aa, 14 kDa |
| 观测分子量 | 18 kDa |
| GenBank蛋白编号 | BC071706 |
| 基因名称 | INSL3 |
| Gene ID (NCBI) | 3640 |
| RRID | AB_2878479 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P51460 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Insulin-like 3 (INSL3), a Leydig cell-specific hormone, is essential for testis descent during foetal life and bone metabolism in adults. Insl3-deficient mice exhibit bilateral undescended testes located high in the abdominal cavity. INSL3 is presumed also to conform to a heterodimeric A-B structure of approximately 6 kDa once released from the producing cells. It is possible that both the mature form of INSL3 (6.6 kDa) and the pro-form(s) of INSL3 (12-14 kDa) are secreted from mammalian Leydig cells (PMID: 19329805).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for INSL3 antibody 17997-1-AP | Download protocol |
| WB protocol for INSL3 antibody 17997-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Exp Mol Pathol Transcriptomic analysis of the HPT axis in a model of oligoasthenozoospermia induced by Adenine in rats | ||
Ecotoxicol Environ Saf Identification of endocrine-disrupting chemicals targeting the genes and pathways of genital anomalies in males |
