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验证数据展示

  • IF Staining of HepG2 using CL488-24766

    IF Staining of HepG2 using CL488-24766

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 INSIG2 antibody (CL488-24766) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-24766

    FC experiment of HepG2 using CL488-24766

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Insig2 Polyclonal Antibody (CL488-24766)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated INSIG2 Polyclonal antibody

INSIG2 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-24766

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

INSIG 2, INSIG-2, Insulin-induced gene 2 protein

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 24766-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-24766 targets INSIG2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag14072

Product name: Recombinant human INSIG2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 79-139 aa of BC022475

Sequence: TASAVIGLLYPCIDRHLGEPHKFKREWSSVMRCVAVFVGINHASAKVDFDNNIQLSLTLAA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 INSIG 2
别名 INSIG 2, INSIG-2, Insulin-induced gene 2 protein
计算分子量 225 aa, 25 kDa
观测分子量 30 kDa
GenBank蛋白编号BC022475
基因名称 INSIG2
Gene ID (NCBI) 51141
RRIDAB_3672772
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y5U4
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

INSIG2 mediates feedback control of cholesterol synthesis by controlling SCAP and HMGCR. INSIG2 may bring HMGCR into ubiquitin-mediated proteasomal degradation. Insig2 protein are endoplasmic reticulum proteins that block the processing of sterol regulatory element binding proteins (SREBPs) by binding to SREBP cleavage-activating protein (SCAP), and thus prevent SCAP from escorting SREBPs to the Golgi. INSIG-2 may play an important role in therapy of hypercholesterolemia.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 INSIG2 antibody CL488-24766Download protocol
IF protocol for CL Plus 488 INSIG2 antibody CL488-24766Download protocol
Standard Protocols
Click here to view our Standard Protocols
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