验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, NIH/3T3 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy INPPL1 Ready-To-Use IHC Kit. INPPL1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87161-1-RR targets INPPL1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | inositol polyphosphate phosphatase-like 1 |
| 别名 | EC:3.1.3.86, Inositol polyphosphate phosphatase-like protein 1, INPPL 1, INPPL-1, Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 2 |
| 计算分子量 | 1258 aa, 139 kDa |
| 观测分子量 | 150 kDa |
| GenBank蛋白编号 | BC140853 |
| 基因名称 | INPPL1 |
| Gene ID (NCBI) | 3636 |
| RRID | AB_3745380 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O15357 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Inositol polyphosphate phosphatase-like protein 1(INPPL1) belongs to the inositol and phosphatidylinositol 5-phosphatase family, also known as SH2 domain-containing inositol 5'-phosphatase 2(SHIP2), is ubiquitous in all organizations. SHIP2 is constitutively tyrosine-phosphorylated in chronic myeloid leukemia progenitor cells. In B cells, SHIP2 was most tyrosine phosphorylated after BCR and FC-γriib crosslinking and was associated with Shc, but not after BCR stimulation alone.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for INPPL1 antibody 87161-1-RR | Download protocol |
| WB protocol for INPPL1 antibody 87161-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
