Various lysates were subjected to SDS PAGE followed by western blot with 87311-1-RR (INPP5F antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87311-1-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 87311-1-RR (INPP5F antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87311-1-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 87311-1-RR (INPP5F antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87311-1-PBS in a different storage buffer formulation.

The mean INPP5F concentration was determined to be 17.30 ng/mL in SH-SY5Y cell extract based on a 1.20 mg/mL extract load, 11.30 ng/mL in HEK-293 cell extract based on a 1.20 mg/mL extract load, 6.40 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load, 7.80 ng/mL in THP-1 cell extract based on a 1.20 mg/mL extract load, and 4.80 ng/mL in HeLa cell extract based on a 1.20 mg/mL extract load.

Sandwich ELISA standard curve of MP02978-2, Human INPP5F Recombinant Matched Antibody Pair - PBS only. 87311-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag39933. 87311-1-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

Biolayer interferometry (BLl) kinetic assays of 87311-1-RR against Human INPP5F were performed. The affinity constant is 0.552 nM.