×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 19694-1-AP (same clone as 19694-1-PBS)

    WB analysis using 19694-1-AP (same clone as 19694-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19694-1-AP (INPP5D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19694-1-PBS in a different storage buffer formulation.

  • IP experiment of Ramos using 19694-1-AP (same clone as 19694-1-PBS)

    IP experiment of Ramos using 19694-1-AP (same clone as 19694-1-PBS)

    IP result of anti-INPP5D (IP:19694-1-AP, 4ug; Detection:19694-1-AP 1:300) with Ramos cells lysate 3600 ug. This data was developed using the same antibody clone with 19694-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 19694-1-AP (same clone as 19694-1-PBS)

    IHC staining of human tonsillitis using 19694-1-AP (same clone as 19694-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19694-1-AP (INPP5D Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19694-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 19694-1-AP (same clone as 19694-1-PBS)

    IHC staining of human tonsillitis using 19694-1-AP (same clone as 19694-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19694-1-AP (INPP5D Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19694-1-PBS in a different storage buffer formulation.

  • WB analysis using 19694-1-AP (same clone as 19694-1-PBS)

    WB analysis using 19694-1-AP (same clone as 19694-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19694-1-AP (INPP5D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19694-1-PBS in a different storage buffer formulation.

INPP5D Polyclonal antibody, PBS Only

INPP5D Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 19694-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

产品信息

19694-1-PBS targets INPP5D in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 inositol polyphosphate-5-phosphatase, 145kDa
别名
计算分子量 133 kDa
观测分子量 145 kDa
GenBank蛋白编号NM_001017915
基因名称 INPP5D
Gene ID (NCBI) 3635
RRIDAB_10667407
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92835
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

INPP5D, also named as SHIP, SHIP1, SIP-145 and hp51CN, belongs to the inositol-1,4,5-trisphosphate 5-phosphatase family. INPP5D is phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways. INPP5D acts as a negative regulator of B-cell antigen receptor signaling. It mediates signaling from the FC-gamma-RIIB receptor (FCGR2B), playing a central role in terminating signal transduction from activating immune/hematopoietic cell receptor systems. INPP5D acts as a negative regulator of myeloid cell proliferation/survival and chemotaxis, mast cell degranulation, immune cells homeostasis, integrin alpha-IIb/beta-3 signaling in platelets and JNK signaling in B-cells. INPP5D regulates proliferation of osteoclast precursors, macrophage programming, phagocytosis and activation and is required for endotoxin tolerance. It is involved in the control of cell-cell junctions, CD32a signaling in neutrophils and modulation of EGF-induced phospholipase C activity. It is a key regulator of neutrophil migration, by governing the formation of the leading edge and polarization required for chemotaxis. It modulates FCGR3/CD16-mediated cytotoxicity in NK cells. It mediates the activin/TGF-beta-induced apoptosis through its Smad-dependent expression. INPP5D may also hydrolyze PtdIns(1,3,4,5)P4, and could thus affect the levels of the higher inositol polyphosphates like InsP6. This antibody is specific to INPP5D.

||
New chat

Able™

正在加载，请稍候...