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验证数据展示

  • WB analysis using 86001-1-RR

    WB analysis using 86001-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86001-1-RR (INPP5A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 86001-1-RR

    Affinity and Kinetic Characterization of 86001-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86001-1-RR against Human INPP5A were performed. The affinity constant is below 1 pM.

INPP5A Recombinant antibody

INPP5A Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 86001-1-RR

产品说明书

CloneNo. 250584C3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

43 kDa inositol polyphosphate 5-phosphatase, 5PTASE, EC:3.1.3.56, Inositol polyphosphate-5-phosphatase A, Type I inositol 1,4,5-trisphosphate 5-phosphatase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, fetal human brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86001-1-RR targets INPP5A in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 INPP5A fusion protein Ag16380 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 inositol polyphosphate-5-phosphatase, 40kDa
别名 43 kDa inositol polyphosphate 5-phosphatase, 5PTASE, EC:3.1.3.56, Inositol polyphosphate-5-phosphatase A, Type I inositol 1,4,5-trisphosphate 5-phosphatase
计算分子量 412 aa, 48 kDa
观测分子量45 kDa
GenBank蛋白编号BC096280
基因名称 INPP5A
Gene ID (NCBI) 3632
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ14642
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

INPP5A (Inositol Polyphosphate-5-Phosphatase A) is a membrane-bound type I inositol 1,4,5-trisphosphate 5-phosphatase that is involved in the mobilization of intracellular calcium ions and the regulation of cellular responses to stimuli. It terminates calcium signaling by hydrolyzing inositol 1,4,5-trisphosphate (InsP3) and inositol 1,3,4,5-tetrakisphosphate (InsP4). INPP5A plays important roles in various cellular processes, including cell proliferation, apoptosis, vesicular transport, and insulin signaling. Additionally, INPP5A is crucial for the survival of cerebellar Purkinje cells. In certain cancers, such as cutaneous squamous cell carcinoma (cSCC) and esophageal squamous cell carcinoma (ESCC), the expression levels of INPP5A are closely related to disease progression and prognosis.

实验方案

Product Specific Protocols
WB protocol for INPP5A antibody 86001-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols