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验证数据展示

  • WB analysis using 85151-4-RR

    WB analysis using 85151-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85151-4-RR (INPP4B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of K562 using 85151-4-RR

    FC experiment of K562 using 85151-4-RR

    1x10^6 K562 were intracellularly stained with 0.25 ug INPP4B Recombinant antibody (85151-4-RR, Clone:242837H2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • IF Staining of MCF-7 using 85151-4-RR

    IF Staining of MCF-7 using 85151-4-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using INPP4B antibody (85151-4-RR, Clone: 242837H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 85151-4-RR

    Affinity and Kinetic Characterization of 85151-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85151-4-RR against Human INPP4B were performed. The affinity constant is 0.563 nM.

INPP4B Recombinant monoclonal antibody

INPP4B Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 85151-4-RR

产品说明书

CloneNo. 242837H2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

242837H2, EC:3.1.3.66, Inositol polyphosphate 4-phosphatase type II, Type II inositol 3,4-bisphosphate 4-phosphatase

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inLNCaP cells, A549 cells, HeLa cells, MCF-7 cells
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inK562

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85151-4-RR targets INPP4B in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag8676

Product name: Recombinant human INPP4B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-53 aa of BC005273

Sequence: MEIKEEGASEEGQHFLPTAQANDPGDCQFTSIQKTPNEPQLEFILDLNQELRD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 inositol polyphosphate-4-phosphatase, type II, 105kDa
别名 242837H2, EC:3.1.3.66, Inositol polyphosphate 4-phosphatase type II, Type II inositol 3,4-bisphosphate 4-phosphatase
计算分子量 53 aa, 6 kDa
观测分子量 100-110 kDa
GenBank蛋白编号BC005273
基因名称 INPP4B
Gene ID (NCBI) 8821
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO15327
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

INPP4B (Inositol polyphosphate 4-phosphatase type II) can catalyze the hydrolysis of the 4-position phosphate of phosphatidylinositol 3,4-bisphosphate, inositol 1,3,4-trisphosphate and inositol 3,4-trisphosphate (PMID: 24070612; 24591580). It also Plays a role in the late stages of macropinocytosis by dephosphorylating phosphatidylinositol 3,4-bisphosphate in membrane ruffles (PMID: 24591580).

实验方案

Product Specific Protocols
FC protocol for INPP4B antibody 85151-4-RRDownload protocol
IF protocol for INPP4B antibody 85151-4-RRDownload protocol
WB protocol for INPP4B antibody 85151-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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