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验证数据展示

  • WB analysis using 29488-1-AP (same clone as 29488-1-PBS)

    WB analysis using 29488-1-AP (same clone as 29488-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 29488-1-AP (INO80B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29488-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse ovary using 29488-1-AP (same clone as 29488-1-PBS)

    IHC staining of mouse ovary using 29488-1-AP (same clone as 29488-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 29488-1-AP (INO80B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29488-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 29488-1-AP (same clone as 29488-1-PBS)

    IF Staining of HepG2 using 29488-1-AP (same clone as 29488-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using INO80B antibody (29488-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 29488-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 29488-1-AP (same clone as 29488-1-PBS)

    WB analysis of HeLa using 29488-1-AP (same clone as 29488-1-PBS)

    WB result of INO80B antibody (29488-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-INO80B transfected HeLa cells. This data was developed using the same antibody clone with 29488-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

INO80B Polyclonal antibody, PBS Only

INO80B Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 29488-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

29488-1-PBS targets INO80B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag30345

Product name: Recombinant human INO80B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 29-265 aa of BC050666

Sequence: HGHGVHKKKHKKHKKKHKKKHHQEEDAGPTQPSPAKPQLKLKIKLGGQVLGTKSVPTFTVIPEGPRSPSPLMVVDNEEEPMEGVPLEQYRAWLDEDSNLSPSPLRDLSGGLGGQEEEEEQRWLDALEKGELDDNGDLKKEINERLLTARQRALLQKARSQPSPMLPLPVAEGCPPPALTEEMLLKREERARKRRLQAARRAEEHKNQTIERLTKTAATSGRGGRGGARGERRGGRA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 INO80 complex subunit B
别名
计算分子量 356 aa, 39 kDa
观测分子量45-50 kDa
GenBank蛋白编号BC050666
基因名称 INO80B
Gene ID (NCBI) 83444
RRIDAB_2918316
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9C086
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

INO80B (INO80 complex subunit B), also known as ZNHIT4 (zinc finger HIT domain-containing protein 4), PAPA1 (PAP-1-associated protein 1), is a subunit of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair. INO80B is localized to the nucleolus. The function of INO80B is to induce growth arrest by haulting the cell cycle at the G1 phase. INO80B expression is controlled at the transcriptional level and is highest at the G0 and G1 phases of the cell cycle. In vitro, INO80B binds PAP-1, a splicing factor, and may play a role in nucleolar complexes that regulate ribosome biogenesis and cell cycle events.

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