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验证数据展示

  • WB analysis using 29488-1-AP

    WB analysis using 29488-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 29488-1-AP (INO80B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse ovary using 29488-1-AP

    IHC staining of mouse ovary using 29488-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 29488-1-AP (INO80B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 29488-1-AP

    IF Staining of HepG2 using 29488-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using INO80B antibody (29488-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • WB analysis of HeLa using 29488-1-AP

    WB analysis of HeLa using 29488-1-AP

    WB result of INO80B antibody (29488-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-INO80B transfected HeLa cells.

  • Featured Product
  • KD/KO验证

INO80B Polyclonal antibody

INO80B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 29488-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

hIes2, HMGA1L4, HMGIYL4, IES2, INO80 complex subunit B, INO80B, PAP 1 associated protein 1, PAP 1BP, PAPA 1, PAPA1, ZNHIT4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, MCF-7 cells
Positive IHC detected inmouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29488-1-AP targets INO80B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse
免疫原 INO80B fusion protein Ag30345 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 INO80 complex subunit B
别名 hIes2, HMGA1L4, HMGIYL4, IES2, INO80 complex subunit B, INO80B, PAP 1 associated protein 1, PAP 1BP, PAPA 1, PAPA1, ZNHIT4
计算分子量 356 aa, 39 kDa
观测分子量45-50 kDa
GenBank蛋白编号BC050666
基因名称 INO80B
Gene ID (NCBI) 83444
RRIDAB_2918316
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9C086
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

INO80B (INO80 complex subunit B), also known as ZNHIT4 (zinc finger HIT domain-containing protein 4), PAPA1 (PAP-1-associated protein 1), is a subunit of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair. INO80B is localized to the nucleolus. The function of INO80B is to induce growth arrest by haulting the cell cycle at the G1 phase. INO80B expression is controlled at the transcriptional level and is highest at the G0 and G1 phases of the cell cycle. In vitro, INO80B binds PAP-1, a splicing factor, and may play a role in nucleolar complexes that regulate ribosome biogenesis and cell cycle events.

实验方案

Product Specific Protocols
WB protocol for INO80B antibody 29488-1-APDownload protocol
IHC protocol for INO80B antibody 29488-1-APDownload protocol
IF protocol for INO80B antibody 29488-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols