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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68064-1-Ig (same clone as 68064-1-PBS)

    WB analysis using 68064-1-Ig (same clone as 68064-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68064-1-Ig (INO80 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68064-1-PBS in a different storage buffer formulation.

  • WB analysis using 68064-1-Ig (same clone as 68064-1-PBS)

    WB analysis using 68064-1-Ig (same clone as 68064-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68064-1-Ig (INO80 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68064-1-PBS in a different storage buffer formulation.

  • FC experiment of PC-12 using 68064-1-Ig (same clone as 68064-1-PBS)

    FC experiment of PC-12 using 68064-1-Ig (same clone as 68064-1-PBS)

    1X10^6 PC-12 cells were intracellularly stained with 0.8 ug Anti-Human INO80 (68064-1-Ig, Clone:1F11C5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.8 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68064-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 68064-1-Ig (same clone as 68064-1-PBS)

    IHC staining of human colon cancer using 68064-1-Ig (same clone as 68064-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68064-1-Ig (INO80 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68064-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 68064-1-Ig (same clone as 68064-1-PBS)

    IHC staining of human colon cancer using 68064-1-Ig (same clone as 68064-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68064-1-Ig (INO80 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68064-1-PBS in a different storage buffer formulation.

INO80 Monoclonal antibody, PBS Only

INO80 Monoclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 68064-1-PBS

产品说明书

CloneNo. 1F11C5

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), Indirect ELISA

别名

hINO80, EC:3.6.4.-, DNA helicase-related protein INO80, DNA helicase-related INO80 complex homolog 1, Chromatin-remodeling ATPase INO80

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68064-1-PBS targets INO80 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 INO80 fusion protein Ag20265 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 INO80 homolog (S. cerevisiae)
别名 hINO80, EC:3.6.4.-, DNA helicase-related protein INO80, DNA helicase-related INO80 complex homolog 1, Chromatin-remodeling ATPase INO80
计算分子量 1556 aa, 177 kDa
观测分子量177 kDa
GenBank蛋白编号BC146785
基因名称 INO80
Gene ID (NCBI) 54617
RRIDAB_2918805
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9ULG1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DNA helicase INO80, also namd as INO80 or hINO80, is a 1,556 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain, and one DBINO domain. INO80 co-localizes with PCMA at replication forks during S-phase in the nucleus and belongs to the SNF2 helicase family. INO80 is a component of the chromatin remolding INO80 complex and is widely expressed in many tissues, such as brain,liver and pancreas. INO80 is involved in transcription regulation.