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验证数据展示

  • IF Staining of A431 using CL488-84148-6

    IF Staining of A431 using CL488-84148-6

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 IMUP antibody (CL488-84148-6, Clone: 241220B4 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated IMUP Recombinant monoclonal antibody

IMUP Recombinant Antibody for IF/ICC
Cat No. CL488-84148-6

产品说明书

CloneNo. 241220B4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

Immortalization up-regulated protein, Hepatocyte growth factor activator inhibitor type 2-related small protein, HAI-2-related small protein, H2RSP, C19orf33

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 84148-6-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-84148-6 targets IMUP in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原

CatNo: Ag22218

Product name: Recombinant human C19orf33 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-85 aa of BC060319

Sequence: MEFDLGAALEPTSQKPGVGAGHGGDPKLSPHKVQGRSEAGAGPGPKASNFRGLGKGRRLTAAPPSSKDTTALPTPAAAPAIRTRM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chromosome 19 open reading frame 33
别名 Immortalization up-regulated protein, Hepatocyte growth factor activator inhibitor type 2-related small protein, HAI-2-related small protein, H2RSP, C19orf33
计算分子量 106 aa, 11 kDa
GenBank蛋白编号BC060319
基因名称 IMUP
Gene ID (NCBI) 64073
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9GZP8
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Immortalization up-regulated protein (IMUP), also known as C19orf33 or H2RSP, was first identified in SV40-immortalized fibroblasts and includes two isoforms, IMUP-1 and IMUP-2 (PMID: 14981917; 35142956). It is found primarily in the nucleus (PMID: 11606055). IMUP is reportedly upregulated in many cancer cells and tissues and its overexpression is associated with tumorigenicity and cell-cycle acceleration (PMID: 16962562; 35142956; 22886722). The apparent molecular determined by SDS-PAGE might be due to post-translational modifications or anomalous migration behavior (PMID: 11080599).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 IMUP antibody CL488-84148-6Download protocol
Standard Protocols
Click here to view our Standard Protocols
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