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验证数据展示

  • WB analysis using 84148-7-RR

    WB analysis using 84148-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84148-7-RR (IMUP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • FC experiment of A431 using 84148-7-RR

    FC experiment of A431 using 84148-7-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug IMUP Recombinant antibody (84148-7-RR, Clone:241220A4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

IMUP Recombinant antibody

IMUP Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84148-7-RR

产品说明书

CloneNo. 241220A4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

Immortalization up-regulated protein, Hepatocyte growth factor activator inhibitor type 2-related small protein, HAI-2-related small protein, H2RSP, C19orf33

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, L02 cells, A549 cells, EC109 cells
Positive FC (Intra) detected inA431 cells, PC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84148-7-RR targets IMUP in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 IMUP fusion protein Ag22218 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chromosome 19 open reading frame 33
别名 Immortalization up-regulated protein, Hepatocyte growth factor activator inhibitor type 2-related small protein, HAI-2-related small protein, H2RSP, C19orf33
计算分子量 106 aa, 11 kDa
观测分子量20-22 kDa
GenBank蛋白编号BC060319
基因名称 IMUP
Gene ID (NCBI) 64073
RRIDAB_3671712
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9GZP8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Immortalization up-regulated protein (IMUP), also known as C19orf33 or H2RSP, was first identified in SV40-immortalized fibroblasts and includes two isoforms, IMUP-1 and IMUP-2 (PMID: 14981917; 35142956). It is found primarily in the nucleus (PMID: 11606055). IMUP is reportedly upregulated in many cancer cells and tissues and its overexpression is associated with tumorigenicity and cell-cycle acceleration (PMID: 16962562; 35142956; 22886722). The apparent molecular determined by SDS-PAGE might be due to post-translational modifications or anomalous migration behavior (PMID: 11080599).

实验方案

Product Specific Protocols
WB protocol for IMUP antibody 84148-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols