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验证数据展示

  • WB analysis of HepG2 using 24738-1-AP

    WB analysis of HepG2 using 24738-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 24738-1-AP (ILVBL Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 24738-1-AP

    IHC staining of human heart using 24738-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24738-1-AP (ILVBL Antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human heart using 24738-1-AP

    IHC staining of human heart using 24738-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24738-1-AP (ILVBL Antibody) at dilution of 1:100 (under 40x lens).

ILVBL Polyclonal antibody

ILVBL Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24738-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

209L8, AHAS, ILV2H, IlvB like protein, ILVBL

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, MCF-7 cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24738-1-AP targets ILVBL in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag21495

Product name: Recombinant human ILVBL protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-632 aa of BC011761

Sequence: METPAAAAPAGSLFPSFLLLACGTLVAALLGAAHRLGLFYQLLHKVDKASVRHGGENVAAVLRAHGVRFIFTLVGGHISPLLVACEKLGIRVVDTRHEVTAVFAADAMARLSGTVGVAAVTAGPGLTNTVTAVKNAQMAQSPILLLGGAASTLLQNRGALQAVDQLSLFRPLCKFCVSVRRVRDIVPTLRAAMAAAQSGTPGPVFVELPVDVLYPYFMVQKEMVPAKPPKGLVGRVVSWYLENYLANLFAGAWEPQPEGPLPLDIPQASPQQVQRCVEILSRAKRPLMVLGSQALLTPTSADKLRAAVETLGVPCFLGGMARGLLGRNHPLHIRENRSAALKKADVIVLAGTVCDFRLSYGRVLSHSSKIIIVDRNREEMLLNSDIFWKPQEAVQGDVGSFVLKLVEGLQGQTWAPDWVEELREADRQKEQTFREKAAMPVAQHLNPVQVLQLVEETLPDNSILVVDGGDFVGTAAHLVQPRGPLRWLDPGAFGTLGVGAGFALGAKLCRPDAEVWCLFGDGAFGYSLIEFDTFVRHKIPVMALVGNDAGWTQISREQVPSLGSNVACGLAYTDYHKAAMGLGARGLLLSRENEDQVVKVLHDAQQQCRDGHPVVVNILIGRTDFRDGSIAV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ilvB (bacterial acetolactate synthase)-like
别名 209L8, AHAS, ILV2H, IlvB like protein, ILVBL
计算分子量 68 kDa
观测分子量 68 kDa
GenBank蛋白编号BC011761
基因名称 ILVBL
Gene ID (NCBI) 10994
RRIDAB_2879699
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDA1L0T0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for ILVBL antibody 24738-1-APDownload protocol
WB protocol for ILVBL antibody 24738-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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