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验证数据展示

  • FC experiment of human PBMCs using CL488-FcA98486-3

    FC experiment of human PBMCs using CL488-FcA98486-3

    1x10^6 human PBMCs were surface stained with CoraLite® Plus 647 Anti-Human IL3RA/CD123, and 5 ul CoraLite® Plus 488 Anti-Human ILT-3/LILRB4 Rabbit RecAb (CL488-FcA98486-3, Clone: 250356B3) or FcZero-rAb® CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-FcA98136, Clone: 240953C9). Cells were not fixed.

  • FC experiment of human PBMCs using CL488-FcA98486-3

    FC experiment of human PBMCs using CL488-FcA98486-3

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human ILT-3/LILRB4 Rabbit RecAb (CL488-FcA98486-3, Clone: 250356B3) (red) or FcZero-rAb® CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-FcA98136, Clone: 240953C9) (blue). Cells were not fixed.

  • Zero Fc receptor binding
  • Site-specific conjugation
  • One universal isotype

FcZero-rAb® CoraLite® Plus 488 Anti-Human ILT-3/LILRB4 Rabbit Recombinant Antibody

ILT-3/LILRB4 FcZero-rAb® Recombinant Antibody for FC
Cat No. CL488-FcA98486-3

产品说明书

CloneNo. 250356B3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

LILRB4, B4, CD85 antigen-like family member K, CD85k, ILT3

缓冲液配方:  PBS, Azide, BSA
PBS, Azide, BSA
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 5 ul per 10^6 cells in a 100 µl suspension
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-FcA98486-3 targets ILT-3/LILRB4 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原

CatNo: Eg1743

Product name: Recombinant Human ILT-3/LILRB4 protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 22-259 aa of AAH26309.1

Sequence: QAGPLPKPTLWAEPGSVISWGNSVTIWCQGTLEAREYRLDKEESPAPWDRQNPLEPKNKARFSIPSMTEDYAGRYRCYYRSPVGWSQPSDPLELVMTGAYSKPTLSALPSPLVTSGKSVTLLCQSRSPMDTFLLIKERAAHPLLHLRSEHGAQQHQAEFPMSPVTSVHGGTYRCFSSHGFSHYLLSHPSDPLELIVSGSLEGPRPSPTRSVSTAAGPEDQPLMPTGSVPHSGLRRHWE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 4
别名 LILRB4, B4, CD85 antigen-like family member K, CD85k, ILT3
计算分子量49kDa
GenBank蛋白编号AAH26309.1
基因名称 LILRB4
Gene ID (NCBI) 11006
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
激发激光Blue laser (488 nm)
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8NHJ6
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

LILRB4 is an inhibitory receptor in the LILR family mainly expressed on normal and malignant human cells of myeloid origin. LILRB4 is activated and subsequently recruits adaptors to cytoplasmic immunoreceptor tyrosine inhibitory motifs to initiate different signaling cascades, thus playing an important role in physiological and pathological conditions, including autoimmune diseases, microbial infections, and cancers.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 ILT-3/LILRB4 antibody CL488-FcA98486-3Download protocol
Standard Protocols
Click here to view our Standard Protocols
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