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验证数据展示

  • WB analysis using 85428-5-RR (same clone as 85428-5-PBS)

    WB analysis using 85428-5-RR (same clone as 85428-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85428-5-RR (ILK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85428-5-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 85428-5-RR (same clone as 85428-5-PBS)

    IF Staining of HeLa using 85428-5-RR (same clone as 85428-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ILK antibody (85428-5-RR, Clone: 242982A5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85428-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85428-5-RR

    Affinity and Kinetic Characterization of 85428-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85428-5-RR against Human ILK were performed. The affinity constant is below 1 pM.

ILK Recombinant monoclonal antibody, PBS Only

ILK Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 85428-5-PBS

产品说明书

CloneNo. 242982A5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, pig

应用

WB, IF/ICC, Indirect ELISA

别名

59 kDa serine/threonine-protein kinase, Beta-integrin-linked kinase, EC:2.7.11.1, ILK 1, ILK 2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85428-5-PBS targets ILK in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, pig samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, pig
免疫原

CatNo: Ag3570

Product name: Recombinant human ILK protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 102-451 aa of BC001554

Sequence: VPLHYACFWGQDQVAEDLVANGALVSICNKYGEMPVDKAKAPLRELLRERAEKMGQNLNRIPYKDTFWKGTTRTRPRNGTLNKHSGIDFKQLNFLTKLNENHSGELWKGRWQGNDIVVKVLKVRDWSTRKSRDFNEECPRLRIFSHPNVLPVLGACQSPPAPHPTLITHWMPYGSLYNVLHEGTNFVVDQSQAVKFALDMARGMAFLHTLEPLIPRHALNSRSVMIDEDMTARISMADVKFSFQCPGRMYAPAWVAPEALQKKPEDTNRRSADMWSFAVLLWELVTREVPFADLSNMEIGMKVALEGLRPTIPPGISPHVCKLMKICMNEDPAKRPKFDMIVPILEKMQD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 integrin-linked kinase
别名 59 kDa serine/threonine-protein kinase, Beta-integrin-linked kinase, EC:2.7.11.1, ILK 1, ILK 2
计算分子量 451 aa, 59 kDa
观测分子量51 kDa
GenBank蛋白编号BC001554
基因名称 ILK
Gene ID (NCBI) 3611
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ13418
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ILK(Integrin-linked protein kinase) is also named as p59, PAT-4, DKFZp686F1765, ILK-1, ILK-2, ILK1, ILK2, p59ILK and belongs to the TKL Ser/Thr protein kinase family. This protein localizes to multiple sites, including the cytoplasm, and imports into the nucleus through sequences in its N-terminus, via active transport mechanisms that involve nuclear pore complexes(PMID: 18635968). ILK performs crucial roles in the control of intestinal cell and crypt-villus axis homeostasis-especially with regard to basement membrane fibronectin deposition-as well as cell proliferation, spreading, and migration(PMID:19885839).

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