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验证数据展示

  • IHC staining of human pancreas cancer using 24926-1-AP

    IHC staining of human pancreas cancer using 24926-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 24926-1-AP (ILK Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 24926-1-AP

    IHC staining of human pancreas cancer using 24926-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 24926-1-AP (ILK Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of mouse heart using 24926-1-AP

    WB analysis of mouse heart using 24926-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 24926-1-AP (ILK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 24926-1-AP

    IF Staining of MCF-7 using 24926-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 24926-1-AP (ILK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

ILK Polyclonal antibody

ILK Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24926-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

ILK, ILK 1, ILK 2, ILK1, ILK2, integrin linked kinase, Integrin linked protein kinase, P59, p59ILK

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse heart tissue, MCF-7 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24926-1-AP targets ILK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse
文献引用反应性mouse
免疫原

CatNo: Ag21494

Product name: Recombinant human ILK protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-452 aa of BC001554

Sequence: MDDIFTQCREGNAVAVRLWLDNTENDLNQGDDHGFSPLHWACREGRSAVVEMLIMRGARINVMNRGDDTPLHLAASHGHRDIVQKLLQYKADINAVNEHGNVPLHYACFWGQDQVAEDLVANGALVSICNKYGEMPVDKAKAPLRELLRERAEKMGQNLNRIPYKDTFWKGTTRTRPRNGTLNKHSGIDFKQLNFLTKLNENHSGELWKGRWQGNDIVVKVLKVRDWSTRKSRDFNEECPRLRIFSHPNVLPVLGACQSPPAPHPTLITHWMPYGSLYNVLHEGTNFVVDQSQAVKFALDMARGMAFLHTLEPLIPRHALNSRSVMIDEDMTARISMADVKFSFQCPGRMYAPAWVAPEALQKKPEDTNRRSADMWSFAVLLWELVTREVPFADLSNMEIGMKVALEGLRPTIPPGISPHVCKLMKICMNEDPAKRPKFDMIVPILEKMQDK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 integrin-linked kinase
别名 ILK, ILK 1, ILK 2, ILK1, ILK2, integrin linked kinase, Integrin linked protein kinase, P59, p59ILK
计算分子量 451 aa, 59 kDa
观测分子量 51 kDa
GenBank蛋白编号BC001554
基因名称 ILK
Gene ID (NCBI) 3611
RRIDAB_2879800
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13418
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ILK(Integrin-linked protein kinase) is also named as p59, PAT-4, DKFZp686F1765, ILK-1, ILK-2, ILK1, ILK2, p59ILK and belongs to the TKL Ser/Thr protein kinase family. This protein localizes to multiple sites, including the cytoplasm, and imports into the nucleus through sequences in its N-terminus, via active transport mechanisms that involve nuclear pore complexes(PMID: 18635968). ILK performs crucial roles in the control of intestinal cell and crypt-villus axis homeostasis-especially with regard to basement membrane fibronectin deposition-as well as cell proliferation, spreading, and migration(PMID:19885839).

实验方案

Product Specific Protocols
IF protocol for ILK antibody 24926-1-APDownload protocol
IHC protocol for ILK antibody 24926-1-APDownload protocol
WB protocol for ILK antibody 24926-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

ACS Appl Mater Interfaces

Charge Reversal Polypyrrole Nanocomplex-Mediated Gene Delivery and Photothermal Therapy for Effectively Treating Papillary Thyroid Cancer and Inhibiting Lymphatic Metastasis.

Authors - Menghong Xu
View Article
WB,IF

Cell Death Discov

Anaerobic metabolism promotes breast cancer survival via Histone-3 Lysine-18 lactylation mediating PPARD axis

Authors - Ying Xu
View Article
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