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验证数据展示

  • WB analysis using 67051-1-Ig

    WB analysis using 67051-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67051-1-Ig (IL-4RA/CD124 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

IL-4RA/CD124 Monoclonal antibody

IL-4RA/CD124 Monoclonal Antibody for WB, ELISA
Cat No. 67051-1-Ig

产品说明书

CloneNo. 1E4C11

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, ELISA

别名

IL4R, IL 4R subunit alpha, IL 4R alpha, IL 4 receptor subunit alpha, IL 4 binding protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, Jurkat cells, MG-63 cells, SCaBER cells, HT-1376 cells, Raji cells, Ramos cells, Daudi cells, HUVEC cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67051-1-Ig targets IL-4RA/CD124 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 IL-4RA/CD124 fusion protein Ag28411 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 interleukin 4 receptor
别名 IL4R, IL 4R subunit alpha, IL 4R alpha, IL 4 receptor subunit alpha, IL 4 binding protein
计算分子量 90 kDa
观测分子量 120 kDa
GenBank蛋白编号NM_000418
基因名称 IL-4R
Gene ID (NCBI) 3566
RRIDAB_2882364
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP24394
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IL-4 receptor alpha chain (IL-4RA), also known as CD124, is a transmembrane glycoprotein expressed on a wide variety of hematopoietic and non-hematopoietic cells. It is a receptor for both IL-4 and IL-13. IL-4RA can complex with the common gamma chain (IL-2R gamma or CD132) and utilizes JAK1 and JAK2 for signal transduction, while complexes of IL-4RA with IL-13RA1 subunit mediate signals via JAK2 and Tyk2. The IL-4 response is involved in promoting Th2 differentiation. The IL-4/IL-13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation.

实验方案

Product Specific Protocols
WB protocol for IL-4RA/CD124 antibody 67051-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols