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CoraLite® Plus 488-conjugated IL-33 Monoclonal antibody

IL-33 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2A9C7

Cat No : CL488-66235

Print datasheet

Synonyms

C9orf26, DKFZp586H0523, DVS27, IL 1F11, IL 33, IL1F11, IL33, IL-33, Interleukin 1 family member 11, interleukin 33, NF HEV, NFEHEV, NFHEV, RP11 575C20.2

验证数据展示

  • IF Staining of HepG2 using CL488-66235

    IF Staining of HepG2 using CL488-66235

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL488-66235 (IL33 antibody) at dilution of 1:100.

经过测试的应用

Positive IF detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66235-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66235 targets IL-33 in IF applications and shows reactivity with human samples.

Tested Applications IF/ICC
Tested Reactivity human
Immunogen IL-33 fusion protein Ag21430 种属同源性预测
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Full Name interleukin 33
Synonyms C9orf26, DKFZp586H0523, DVS27, IL 1F11, IL 33, IL1F11, IL33, IL-33, Interleukin 1 family member 11, interleukin 33, NF HEV, NFEHEV, NFHEV, RP11 575C20.2
Calculated Molecular Weight 30 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC047085
Gene Symbol IL33
Gene ID (NCBI) 90865
RRIDAB_2883291
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodThiophilic affinity chromatograph
UNIPROT IDO95760
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IL-33 is an IL-1 family cytokine and nuclear alarmin, is constitutively expressed in epithelial barrier tissues and human blood vessels. IL-33 is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. It also acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 IL-33 antibody CL488-66235Download protocol
Standard Protocols
Click here to view our Standard Protocols